胰腺导管腺癌诱导的外周淋巴细胞亚群异常分布调节总生存期。

Abnormal distribution of peripheral lymphocyte subsets induced by PDAC modulates overall survival.

作者信息

Xu Yong-Feng, Lu Yu, Cheng He, Shi Si, Xu Jin, Long Jiang, Liu Liang, Liu Chen, Yu Xianjun

机构信息

Department of Pancreatic and Hepatobiliary Surgery, Fudan University Shanghai Cancer Center, No. 270 DongAn Road, Shanghai 200032, PR China; Department of Oncology, Shanghai Medical College, Fudan University, No. 270 DongAn Road, Shanghai 200032, PR China; Pancreatic Cancer Institute, Fudan University, No. 270 DongAn Road, Shanghai 200032, PR China.

Department of Pancreatic and Hepatobiliary Surgery, Fudan University Shanghai Cancer Center, No. 270 DongAn Road, Shanghai 200032, PR China; Pancreatic Cancer Institute, Fudan University, No. 270 DongAn Road, Shanghai 200032, PR China.

出版信息

Pancreatology. 2014 Jul-Aug;14(4):295-301. doi: 10.1016/j.pan.2014.05.797. Epub 2014 Jun 11.

DOI:10.1016/j.pan.2014.05.797

PMID:25062880

Abstract

BACKGROUND/OBJECTIVES: The impairment of the immune system is prevalent in patients with malignancies, including pancreatic ductal adenocarcinoma (PDAC). The present study aimed to evaluate alternations of peripheral lymphocyte subsets in patients with PDAC, and also to assess the prognostic value of observed changes.

METHODS

We recruited 160 consecutive PDAC patients who had undergone radical surgical resection between 2010 and 2013. To investigate the prognostic factors, we detected the peripheral lymphocyte subsets in PDAC by flow cytometry, including T cells (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD8(+)CD28(+)), regulatory T cells (Tregs, CD4(+)CD25(+)CD127(-)), natural killer cells (NK cells, CD3(-)CD56(+)) and B cells (CD19(+)). We also evaluated the clinical and pathological features of these patients. Survival analysis was performed by univariate and multivariate analyses.

RESULTS

Our results indicated the profile of peripheral lymphocyte subsets undergone profound changes in PDAC patients. Univariate and multivariate analysis indicated the levels of peripheral lymphocyte subsets (CD19(+) B cells, Tregs and CD8(+)CD28(+) T cells) were independent predictors for overall survival. The results also suggested that the systemic impairment of immune system in patients with PDAC, was reversed when primary tumor was removed.

CONCLUSIONS

The present study provided some evidences that the impairment of host immunity induced by PDAC may play a role in the survival of patients.

摘要

背景/目的：免疫系统损害在包括胰腺导管腺癌（PDAC）在内的恶性肿瘤患者中普遍存在。本研究旨在评估PDAC患者外周淋巴细胞亚群的变化，并评估所观察到的变化的预后价值。

方法

我们招募了2010年至2013年间连续接受根治性手术切除的160例PDAC患者。为了研究预后因素，我们通过流式细胞术检测了PDAC患者外周淋巴细胞亚群，包括T细胞（CD3(+)、CD3(+)CD4(+)、CD3(+)CD8(+)、CD8(+)CD28(+)）、调节性T细胞（Tregs，CD4(+)CD25(+)CD127(-)）、自然杀伤细胞（NK细胞，CD3(-)CD56(+)）和B细胞（CD19(+)）。我们还评估了这些患者的临床和病理特征。通过单因素和多因素分析进行生存分析。