Ratcliffe M J
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
J Exp Med. 1989 Oct 1;170(4):1165-73. doi: 10.1084/jem.170.4.1165.
The avian bursa of Fabricius represents a site for the generation of antibody diversity. Transfer of neonatal bursal cells into cyclophosphamide-treated neonatal chickens results in reconstitution of recipient bursae with donor-derived bursal stem cells. These stem cells express cell surface IgM and, under conditions of limiting donor bursal stem cell numbers, each reconstituted bursal follicle is colonized by a single precursor cell. The expression of an Ig VH idiotype, CVH-1, was found to be heterogenous within such clonal follicles. The diversity generated within the bursa is subsequently found within the peripheral B cell compartment. Thus, the generation of functional Ig H chain diversity is shown to occur subsequent to Ig H chain rearrangement and expression.
禽类法氏囊是产生抗体多样性的场所。将新生雏鸡的法氏囊细胞移植到经环磷酰胺处理的新生鸡体内,会导致受体法氏囊被供体来源的法氏囊干细胞重建。这些干细胞表达细胞表面IgM,在供体法氏囊干细胞数量有限的情况下,每个重建的法氏囊滤泡由单个前体细胞定植。发现在这种克隆滤泡内Ig VH独特型CVH-1的表达是异质的。随后在外周B细胞区室中发现法氏囊内产生的多样性。因此,功能性Ig H链多样性的产生显示在Ig H链重排和表达之后发生。