间充质干细胞通过诱导骨髓来源的CD11b⁺Gr1⁺细胞的抑制功能促进小鼠乳腺肿瘤进展
[Mesenchymal stem cells promote mouse breast tumor progression by inducing the suppressive function of myeloid-derived CD11b⁺ Gr1⁺ cells].
作者信息
Hu Xiaoyu, Luo Yuechen, Zhou Yushan, Zhao Dan, Wu Wantong, Han Zhongchao, Feng Xiaoming
机构信息
State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Aug;30(8):785-8.
OBJECTIVE
To investigate the role of mesenchymal stem cells (MSCs) in tumor progression by inducing immuno-suppressive function of myeloid-derived CD11b⁺ Gr1⁺ cells (BM-CD11b⁺ Gr1⁺ cells).
METHODS
After co-cultured with MSCs, the immunophenotypes of BM-CD11b⁺ Gr1⁺ cells were tested by flow cytometry. The influence of MSCs on CD11b⁺ Gr1⁺ cells was evaluated by T cell proliferation assay after cultured with or without MSCs. The mouse 4T1 breast tumor model was established to explore the effect on tumor growth.
RESULTS
MSCs promoted the survival of CD11b⁺ Gr1⁺ cells and induced the generation of CD11b⁺ Gr1⁺ cells from CD11b⁻ Gr1⁻ cells sorted from bone marrow. MSCs also enhanced the ability of BM-CD11b⁺ Gr1⁺ cells to suppress T cell proliferation and activation. CD11b⁺ Gr1⁺ cells after co-cultured with MSCs promoted 4T1 tumor growth and accelerated death of tumor-bearing mice.
CONCLUSION
MSCs can promote tumor progression by inducing suppressive function of myeloid-derived CD11b⁺ Gr1⁺ cells from bone marrow.
目的
通过诱导骨髓来源的CD11b⁺Gr1⁺细胞（BM-CD11b⁺Gr1⁺细胞）的免疫抑制功能，探讨间充质干细胞（MSCs）在肿瘤进展中的作用。
方法
与MSCs共培养后，通过流式细胞术检测BM-CD11b⁺Gr1⁺细胞的免疫表型。在有或无MSCs培养的情况下，通过T细胞增殖试验评估MSCs对CD11b⁺Gr1⁺细胞的影响。建立小鼠4T1乳腺肿瘤模型以探讨其对肿瘤生长的影响。
结果
MSCs促进了CD11b⁺Gr1⁺细胞的存活，并诱导了从骨髓中分选的CD11b⁻Gr1⁻细胞产生CD11b⁺Gr1⁺细胞。MSCs还增强了BM-CD11b⁺Gr1⁺细胞抑制T细胞增殖和激活的能力。与MSCs共培养后的CD11b⁺Gr1⁺细胞促进了4T1肿瘤生长并加速了荷瘤小鼠的死亡。
结论
MSCs可通过诱导骨髓来源的髓系CD11b⁺Gr1⁺细胞的抑制功能促进肿瘤进展。
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