基于量子点/双酶的 H2O2 敏感荧光生物传感器用于尿酸的灵敏检测
A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes.
机构信息
Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia.
出版信息
Biosens Bioelectron. 2015 May 15;67:129-33. doi: 10.1016/j.bios.2014.07.056. Epub 2014 Jul 30.
A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.
一种新型的光学检测系统，由尿酸酶/HRP-CdS 量子点（QDs）组成，用于测定尿液样品中的尿酸。QDs 被用作指示剂，揭示尿酸酶和 HRP（辣根过氧化物酶）的酶促反应导致的系统荧光性质，该反应涉及将尿酸氧化为别嘌呤醇和过氧化氢。产生的过氧化氢能够猝灭 QDs 的荧光，其与尿酸浓度成正比。该系统在尿酸酶和 HRP 的比例为 5U:5U 和 pH 值为 7.0 时表现出足够的活性。该系统对尿酸的线性范围在 125-1000µM 之间，检测限为 125µM。
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