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纽蛋白中Y1065位点的磷酸化介导肌动蛋白成束、细胞铺展以及对力的机械反应。

Phosphorylation at Y1065 in vinculin mediates actin bundling, cell spreading, and mechanical responses to force.

作者信息

Tolbert Caitlin E, Thompson Peter M, Superfine Richard, Burridge Keith, Campbell Sharon L

机构信息

Department of Cell Biology and Physiology, ‡Department of Biochemistry and Biophysics, §Graduate Molecular and Cellular Biophysics Program, ∥Department of Physics and Astronomy, and ⊥the Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599, United States.

出版信息

Biochemistry. 2014 Sep 2;53(34):5526-36. doi: 10.1021/bi500678x. Epub 2014 Aug 21.

DOI:10.1021/bi500678x
PMID:25115937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4151700/
Abstract

Vinculin is an essential structural adaptor protein that localizes to sites of adhesion and is involved in a number of cell processes including adhesion, spreading, motility, force transduction, and cell survival. The C-terminal vinculin tail domain (Vt) contains the necessary structural components to bind and cross-link actin filaments. Actin binding to Vt induces a conformational change that promotes dimerization through the C-terminal hairpin of Vt and enables actin filament cross-linking. Here we show that Src phosphorylation of Y1065 within the C-terminal hairpin regulates Vt-mediated actin bundling and provide a detailed characterization of Y1065 mutations. Furthermore, we show that phosphorylation at Y1065 plays a role in cell spreading and the response to the application of mechanical force.

摘要

纽蛋白是一种重要的结构衔接蛋白，定位于黏附位点，并参与包括黏附、铺展、迁移、力转导和细胞存活在内的多种细胞过程。纽蛋白的C末端尾部结构域（Vt）包含结合和交联肌动蛋白丝所需的结构成分。肌动蛋白与Vt的结合会诱导构象变化，该变化通过Vt的C末端发夹结构促进二聚化，并实现肌动蛋白丝的交联。在这里，我们表明C末端发夹结构内Y1065位点的Src磷酸化调节Vt介导的肌动蛋白成束，并对Y1065突变进行了详细表征。此外，我们表明Y1065位点的磷酸化在细胞铺展和对施加机械力的反应中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/249a378137d8/bi-2014-00678x_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/ae94389c7f7b/bi-2014-00678x_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/30d79916ae8e/bi-2014-00678x_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/c29533b19cee/bi-2014-00678x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/70030d992d4e/bi-2014-00678x_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/249a378137d8/bi-2014-00678x_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/ae94389c7f7b/bi-2014-00678x_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/30d79916ae8e/bi-2014-00678x_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/c29533b19cee/bi-2014-00678x_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/70030d992d4e/bi-2014-00678x_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f33/4151700/249a378137d8/bi-2014-00678x_0004.jpg

相似文献

[1]
Phosphorylation at Y1065 in vinculin mediates actin bundling, cell spreading, and mechanical responses to force.

Biochemistry. 2014-9-2

[2]
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[3]
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[4]
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[5]
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J Mol Biol. 2019-3-5

[6]
Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.

Structure. 2014-5-6

[7]
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J Biol Chem. 2010-5-18

[8]
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Structure. 2019-8-15

[9]
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Biochem J. 2015-2-1

[10]
Vinculin phosphorylation at residues Y100 and Y1065 is required for cellular force transmission.

J Cell Sci. 2015-9-15

引用本文的文献

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J Cell Sci. 2024-9-15

[2]
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Nanoscale Res Lett. 2021-9-15

[3]
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[4]
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[5]
Distinct Binding Modes of Vinculin Isoforms Underlie Their Functional Differences.

Structure. 2019-8-15

[6]
Stiffness-dependent motility and proliferation uncoupled by deletion of CD44.

Sci Rep. 2017-11-28

[7]
Zygotic vinculin is not essential for embryonic development in zebrafish.

PLoS One. 2017-8-2

[8]
Vinculin in cell-cell and cell-matrix adhesions.

Cell Mol Life Sci. 2017-8

[9]
A Structural Model for Vinculin Insertion into PIP-Containing Membranes and the Effect of Insertion on Vinculin Activation and Localization.

Structure. 2017-2-7

[10]
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Cell Mol Life Sci. 2017-1

本文引用的文献

[1]
Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.

Structure. 2014-5-6

[2]
Vinculin phosphorylation at Tyr1065 regulates vinculin conformation and tension development in airway smooth muscle tissues.

J Biol Chem. 2013-12-13

[3]
CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics.

Cell Mol Life Sci. 2013-8-25

[4]
In situ mechanotransduction via vinculin regulates stem cell differentiation.

Stem Cells. 2013-11

[5]
Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth.

J Cell Biol. 2013-7-8

[6]
The interaction of vinculin with actin.

PLoS Comput Biol. 2013-4-25

[7]
Vinculin regulation of F-actin bundle formation: what does it mean for the cell?

Cell Adh Migr. 2013-1-10

[8]
From mechanical force to RhoA activation.

Biochemistry. 2012-9-10

[9]
NMR View: A computer program for the visualization and analysis of NMR data.

J Biomol NMR. 1994-9

[10]
Phosphorylation primes vinculin for activation.

Biophys J. 2012-5-2

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