Gli1和Wnt2B的表达与胰腺癌的进展及临床结果相关。
Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer.
作者信息
Jiang Hua, Li Fengru, He Chengzhi, Wang Xuebin, Li Qingqing, Gao Hengjun
机构信息
Department of Geriatrics, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
Department of Gastroenterology, Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine Shanghai 200065, China.
出版信息
Int J Clin Exp Pathol. 2014 Jun 15;7(7):4531-8. eCollection 2014.
OBJECTIVE
The aim of this study was to investigate the expression and clinical significance of Gli1 and Wnt2B in pancreatic cancer.
METHODS
We have constructed a formalin-fixed paraffin embedded pancreatic tissue microarrays 180 cylindrical tissue cores of human pancreatic cancer and its paracancerous nonmalignant pancreatic specimens (NMPs) from 90 patients. Levels of Gli1 and Wnt2B were measured by immunohistochemistry. We analyzed the correlations between the expression of these factors and clinicopathological parameters including prognosis.
RESULTS
The expressions of both Gli1 and Wnt2B in human pancreatic cancer tissues were significantly higher than those of normal pancreatic tissues (P=0.000, P=0.004 respectively). The analysis showed that the high cytoplasmic expression levels of Gli1 in pancreatic cancer tissues had significant correlation with lymph node metastasis (P=0.036) and Wnt2B had significant correlation with perineural invasion (P=0.045). Gli1 and Wnt2B have no positive correlation. Survival analysis by Kaplan-Meier demonstrated that elevated Wnt2B expression in cancer tissue predicted worse overall survival (OS) compared with group in lower expression (P=0.024). No correlation was found between the expression of Gli1 and overall survival of pancreatic cancer patients (P>0.05).
CONCLUSIONS
In conclusion, these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer, and the high expression of Wnt2B might be associated with poor prognosis.
目的
本研究旨在探讨Gli1和Wnt2B在胰腺癌中的表达及其临床意义。
方法
我们构建了包含来自90例患者的180个圆柱形组织芯的人胰腺癌及其癌旁非恶性胰腺标本（NMPs）的福尔马林固定石蜡包埋胰腺组织微阵列。通过免疫组织化学检测Gli1和Wnt2B的水平。我们分析了这些因子的表达与包括预后在内的临床病理参数之间的相关性。
结果
人胰腺癌组织中Gli1和Wnt2B的表达均显著高于正常胰腺组织（分别为P = 0.000，P = 0.004）。分析表明，胰腺癌组织中Gli1的高细胞质表达水平与淋巴结转移显著相关（P = 0.036），Wnt2B与神经周围浸润显著相关（P = 0.045）。Gli1和Wnt2B无正相关。Kaplan-Meier生存分析表明，与低表达组相比，癌组织中Wnt2B表达升高预示总体生存率（OS）更差（P = 0.024）。未发现Gli1的表达与胰腺癌患者的总体生存率之间存在相关性（P>0.05）。
结论
总之，这些结果表明Gli1和Wnt2B的高表达水平可能在胰腺癌的肿瘤发生过程中起关键作用，并且Wnt2B的高表达可能与预后不良有关。
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