Rao Suresh Ranga, Balaji T M, Prakash P S G, Lavu Vamsi
Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, No. 1 Ramachandra Nagar, Porur, Chennai 600 116, Tamil Nadu, India.
Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, No. 1 Ramachandra Nagar, Porur, Chennai 600 116, Tamil Nadu, India.
Prostaglandins Other Lipid Mediat. 2014 Oct;113-115:69-74. doi: 10.1016/j.prostaglandins.2014.07.003. Epub 2014 Aug 12.
This study was carried out to immuno-localize and estimate the levels of cyclooxygenase 1 and 2 in human gingival tissue samples from healthy individuals, chronic periodontitis patients and patients with cyclosporine induced gingival overgrowth.
Group I consisted of individuals with healthy gingiva (n=6), Group II - cyclosporine induced gingival overgrowth (n=9) and Group III - chronic periodontitis patients (n=6). Gingival tissue samples were collected from subjects of all the three groups. COX-1, COX-2 levels were estimated in tissue homogenates by enzyme activity assay. Immuno-localization for COX-1 and COX-2 was also done in sections of gingival tissue.
The study results demonstrated a significantly higher mean levels of COX-1 and 2 in drug induced gingival overgrowth samples (p<0.05). COX-1 and COX-2 was localized to epithelium and connective tissue in human gingival tissue sections from cyclosporine induced gingival overgrowth.
Cyclooxygenase enzymes appear to be potential mediators involved in the pathogenesis of cyclosporine induced gingival overgrowth.
本研究旨在对健康个体、慢性牙周炎患者以及环孢素诱导性牙龈增生患者的人牙龈组织样本中的环氧化酶1和2进行免疫定位并评估其水平。
第一组由牙龈健康的个体组成（n = 6），第二组为环孢素诱导性牙龈增生患者（n = 9），第三组为慢性牙周炎患者（n = 6）。从所有三组受试者中采集牙龈组织样本。通过酶活性测定法评估组织匀浆中的COX - 1、COX - 2水平。还对牙龈组织切片进行了COX - 1和COX - 2的免疫定位。
研究结果表明，药物诱导性牙龈增生样本中COX - 1和2的平均水平显著更高（p < 0.05）。在环孢素诱导性牙龈增生患者的人牙龈组织切片中，COX - 1和COX - 2定位于上皮和结缔组织。
环氧化酶似乎是参与环孢素诱导性牙龈增生发病机制的潜在介质。
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