评估肺神经内分泌肿瘤中 napsin A、TTF-1、p63、p40 和 CK5/6 免疫组化染色的应用。
Evaluation of napsin A, TTF-1, p63, p40, and CK5/6 immunohistochemical stains in pulmonary neuroendocrine tumors.
机构信息
From the Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis;
Department of Pathology, University of Michigan, Ann Arbor; and.
出版信息
Am J Clin Pathol. 2014 Sep;142(3):320-4. doi: 10.1309/AJCPGA0IUA8BHQEZ.
OBJECTIVE
A panel of immunohistochemical (IHC) stains frequently used to subclassify non-small cell lung cancers (NSCLCs) includes napsin A, TTF-1, CK5/6, p40, and p63. The expression profiles of these stains in neuroendocrine tumors have not been systematically evaluated.
METHOD
Sixty-eight resected pulmonary neuroendocrine tumors, including 52 typical carcinoids (TCs), eight atypical carcinoids (ACs), seven small cell carcinomas (SCLCs) and one large cell neuroendocrine carcinoma (LCNEC), were stained for napsin A, TTF-1, p63, p40, and CK5/6. Tumors were scored as positive (>1% tumor cells reactive) or negative, and percentage of reactive tumor cells was recorded.
RESULTS
Napsin A, p63, p40, and CK5/6 were consistently negative in neuroendocrine tumors. TTF-1 was positive in 17 of 52 TCs, 4 of 8 ACs, 5 of 7 SCLCs, and 0 of 1 LCNECs.
CONCLUSION
Pulmonary neuroendocrine tumors have a distinct but nonspecific profile on IHC panel commonly applied to subclassify NSCLCs. They are napsin A-/p40-/p63-/CK5/6-/TTF-1±. Recognizing this profile may have value in separating neuroendocrine tumors from NSCLCs.
目的
常用于非小细胞肺癌（NSCLC）分类的免疫组织化学（IHC）染色小组包括天冬氨酸蛋白酶 A、甲状腺转录因子-1（TTF-1）、细胞角蛋白 5/6（CK5/6）、p40 和 p63。这些染色在神经内分泌肿瘤中的表达谱尚未得到系统评估。
方法
对 68 例切除的肺神经内分泌肿瘤进行免疫组化染色，包括 52 例典型类癌（TC）、8 例非典型类癌（AC）、7 例小细胞癌（SCLC）和 1 例大细胞神经内分泌癌（LCNEC），用于检测天冬氨酸蛋白酶 A、TTF-1、p63、p40 和 CK5/6。肿瘤评分阳性（>1%肿瘤细胞反应）或阴性，并记录反应性肿瘤细胞的百分比。
结果
神经内分泌肿瘤中天冬氨酸蛋白酶 A、p63、p40 和 CK5/6 始终为阴性。TTF-1 在 52 例 TC 中的 17 例、8 例 AC 中的 4 例、7 例 SCLC 中的 5 例和 1 例 LCNEC 中均为阳性。
结论
肺神经内分泌肿瘤在常用于分类 NSCLC 的 IHC 小组中具有独特但非特异性的特征。它们是天冬氨酸蛋白酶 A-/-/p40-/-/p63-/-/CK5/6-/-/TTF-1±。识别这种特征可能有助于将神经内分泌肿瘤与 NSCLC 区分开来。
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