PDE7A1 水解 cCMP。
PDE7A1 hydrolyzes cCMP.
机构信息
Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; Core Facility Mass Spectrometry and Metabolomics, Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
出版信息
FEBS Lett. 2014 Sep 17;588(18):3469-74. doi: 10.1016/j.febslet.2014.08.005. Epub 2014 Aug 13.
The degradation and biological role of the cyclic pyrimidine nucleotide cCMP is largely elusive. We investigated nucleoside 3',5'-cyclic monophosphate (cNMP) specificity of six different recombinant phosphodiesterases (PDEs) by using a highly-sensitive HPLC-MS/MS detection method. PDE7A1 was the only enzyme that hydrolyzed significant amounts of cCMP. Enzyme kinetic studies using purified GST-tagged truncated PDE7A1 revealed a cCMP KM value of 135 ± 19 μM. The Vmax for cCMP hydrolysis reached 745 ± 27 nmol/(minmg), which is about 6-fold higher than the corresponding velocity for adenosine 3',5'-cyclic monophosphate (cAMP) degradation. In summary, PDE7A is a high-speed and low-affinity PDE for cCMP.
环嘧啶核苷酸 cCMP 的降解和生物学作用在很大程度上难以捉摸。我们通过使用高度敏感的 HPLC-MS/MS 检测方法，研究了六种不同重组磷酸二酯酶 (PDE) 的核苷 3'，5'-环单磷酸 (cNMP) 特异性。PDE7A1 是唯一大量水解 cCMP 的酶。使用纯化的 GST 标记的截断 PDE7A1 进行的酶动力学研究表明，cCMP 的 KM 值为 135±19μM。cCMP 水解的 Vmax 达到 745±27nmol/(minmg)，约为腺苷 3'，5'-环单磷酸 (cAMP) 降解的相应速度的 6 倍。总之，PDE7A 是一种对 cCMP 具有高速和低亲和力的 PDE。
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