Peng Xi, Zhang Keying, Bai Shiping, Ding Xuemei, Zeng Qiufeng, Yang Jun, Fang Jing, Chen Kejie
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan 625014, China.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, China.
Int J Environ Res Public Health. 2014 Aug 20;11(8):8567-80. doi: 10.3390/ijerph110808567.
The purpose of this study was to evaluate the effects of corn naturally contaminated with aflatoxin B1 and aflatoxin B2 on pathological lesions, apoptosis, cell cycle phases and T lymphocyte subsets of spleen, and to provide an experimental basis for understanding the mechanism of aflatoxin-induced immunosuppression. A total of 900 COBB500 male broilers were randomly allocated into five groups with six replicates per group and 30 birds per replicate. The experiment lasted for 6 weeks and the five dietary treatments consisted of control, 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups. The histopathological spleen lesions from the contaminated corn groups was characterized as congestion of red pulp, increased necrotic cells and vacuoles in the splenic corpuscle and periarterial lymphatic sheath. The contaminated corn intake significantly increased relative weight of spleen, percentages of apoptotic splenocytes, induced cell cycle arrest of splenocytes, increased the percentages of CD3+CD8+ T cells and decreased the ratios of CD3+CD4+ to CD3+CD8+. The results suggest that AFB-induced immunosuppression maybe closely related to the lesions of spleen.
本研究旨在评估天然受黄曲霉毒素B1和黄曲霉毒素B2污染的玉米对脾脏病理损伤、细胞凋亡、细胞周期阶段及T淋巴细胞亚群的影响，为了解黄曲霉毒素诱导免疫抑制的机制提供实验依据。将900只COBB500雄性肉鸡随机分为五组，每组六个重复，每个重复30只鸡。实验持续6周，五种日粮处理包括对照组、25%污染玉米组、50%污染玉米组、75%污染玉米组和100%污染玉米组。受污染玉米组的脾脏组织病理学损伤表现为红髓充血、脾小体和动脉周围淋巴鞘中坏死细胞和空泡增多。摄入受污染玉米显著增加了脾脏相对重量、凋亡脾细胞百分比，诱导脾细胞细胞周期停滞，增加了CD3+CD8+T细胞百分比，并降低了CD3+CD4+与CD3+CD8+的比值。结果表明，AFB诱导的免疫抑制可能与脾脏损伤密切相关。
