Rashidipour Marzieh, Heydari Rouhollah, Feizbakhsh Alireza, Hashemi Payman
J AOAC Int. 2014 Jul-Aug;97(4):1109-13. doi: 10.5740/jaoacint.13-209.
Matrix solid-phase dispersion (MSPD) methodology as a quick and easy extraction method has been developed to extract oleuropein from Olea europaea leaves. This method has been compared with conventional maceration and Soxhlet extraction (SOXE) methods. Variables affecting MSPD, such as the sorbent material, the ratio of sample to sorbent material, elution solvent, and volume of the elution solvent, have been fully evaluated and optimized. Oleuropein was successfully extracted by silica sorbent with 350 microL of dichloromethane-methanol (70 + 30, v/v) as the elution solvent. The calibration curve showed good linearity (r2 = 0.9985) and precision (RSD <4.5%) in the concentration range of 0.5-100 microg/mL for oleuropein. The LOD and LOQ were 0.14 and 0.5 microg/mL, respectively. The recoveries were in the range of 88.0-94.0% with RSD values ranging from 6.0 to 9.8%. The extraction yields obtained by the MSPD method were comparable with those obtained by the maceration and SOXE methods. The reported MSPD extraction method was revealed to be eco-friendly, simpler, and faster than conventional methods used to quantify oleuropein in O. europaea leaves.
基质固相分散（MSPD）方法作为一种快速简便的提取方法，已被开发用于从油橄榄叶中提取橄榄苦苷。该方法已与传统的浸渍法和索氏提取（SOXE）法进行了比较。对影响MSPD的变量，如吸附剂材料、样品与吸附剂材料的比例、洗脱溶剂以及洗脱溶剂的体积，进行了全面评估和优化。使用硅胶吸附剂，以350微升二氯甲烷 - 甲醇（70 + 30，v/v）作为洗脱溶剂，成功提取了橄榄苦苷。校准曲线在橄榄苦苷浓度范围为0.5 - 100微克/毫升时显示出良好的线性（r2 = 0.9985）和精密度（RSD <4.5%）。检测限（LOD）和定量限（LOQ）分别为0.14和0.5微克/毫升。回收率在88.0 - 94.0%范围内，相对标准偏差（RSD）值在6.0至9.8%之间。MSPD方法获得的提取率与浸渍法和SOXE方法获得的提取率相当。所报道的MSPD提取方法被证明比用于定量油橄榄叶中橄榄苦苷的传统方法更环保、更简单且更快。
