Gao Yue, Sun Zhan-Yi, Huang Zhi-Hua, Chen Pu-Guang, Chen Yong-Xiang, Zhao Yu-Fen, Li Yan-Mei
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University (P.R. China).
Chemistry. 2014 Oct 13;20(42):13541-6. doi: 10.1002/chem.201404013. Epub 2014 Aug 25.
A novel noncovalent strategy to construct chemically synthesized vaccines has been designed to trigger a robust immune response and to dramatically improve the efficiency of vaccine preparation. Glycosylated MUC1 tripartite vaccines were constructed through host-guest interactions with cucurbit[8]uril. These vaccines elicited high levels of IgG antibodies that were recognized by transformed cells and induced the secretion of cytokines. The antisera also mediated complement-dependent cytotoxicity. This noncovalent strategy with good suitability, scalability, and feasibility can be applied as a universal strategy for the construction of chemically synthesized vaccines.
一种构建化学合成疫苗的新型非共价策略已被设计出来，以引发强烈的免疫反应并显著提高疫苗制备效率。通过与葫芦[8]脲的主客体相互作用构建了糖基化MUC1三方疫苗。这些疫苗引发了高水平的IgG抗体，这些抗体可被转化细胞识别并诱导细胞因子分泌。抗血清还介导补体依赖性细胞毒性。这种具有良好适用性、可扩展性和可行性的非共价策略可作为构建化学合成疫苗的通用策略。
