基于番鸭细小病毒非结构蛋白表位的间接ELISA检测方法的建立，用于区分番鸭的感染和免疫状态。

Development of an indirect ELISA with epitope on nonstructural protein of Muscovy duck parvovirus for differentiating between infected and vaccinated Muscovy ducks.

作者信息

Yan B, Ma J-Z, Yu T-F, Shao S-L, Li M, Fan X-D

机构信息

College of Wildlife Resources, Northeast Forestry University, Harbin, China; College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, China.

出版信息

Lett Appl Microbiol. 2014 Dec;59(6):631-5. doi: 10.1111/lam.12323. Epub 2014 Sep 26.

DOI:10.1111/lam.12323

PMID:25163937

Abstract

UNLABELLED

The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) based on epitope AA503-509 (RANEPKE), which is on nonstructural protein of Muscovy duck parvovirus (MDPV). Sera (100) from negative and vaccinated Muscovy ducks were compared with infected sera (240) to establish the cut-off value of this i-ELISA. There was a significant difference between the positive and negative populations (P < 0·05). The adoption of this positive-negative threshold value for this i-ELISA assay resulted in specificity of 98·0%. This i-ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.

SIGNIFICANCE AND IMPACT OF THE STUDY

In this study, we developed an i-ELISA based on epitope AA503-509 (RANEPKE), which is on nonstructural protein of MDPV. This i-ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.

摘要

未标记

本研究的目的是基于番鸭细小病毒（MDPV）非结构蛋白上的表位AA503 - 509（RANEPKE）开发一种间接酶联免疫吸附测定法（i - ELISA）。将来自阴性和接种疫苗的番鸭的100份血清与240份感染血清进行比较，以确定该i - ELISA的临界值。阳性和阴性群体之间存在显著差异（P < 0·05）。采用该i - ELISA检测的这种阳性 - 阴性阈值，其特异性为98·0%。该i - ELISA可作为一种诊断工具，用于区分感染的番鸭和接种灭活病毒疫苗的番鸭。