Niculescu F, Rus H G, Ionescu D, Maican A, Petcovici M, Malide D, Răhăian R
Arch Roum Pathol Exp Microbiol. 1989 Jul-Sep;48(3):227-35.
Anti-carcinoembryonic (CEA) polyclonal antibodies in sheep and rabbits were raised using purified CEA from acid extracts of human colon adenocarcinoma. CEA was purified by gel filtration on Sepharose 4B CL and chromatography on DEAE-Sephadex A50. The antiserum was adsorbed with human serum and perchloric acid extract from normal colon. Anti-CEA IgG was purified from monospecific antiserum by ion-exchange chromatography and its specificity was tested on cryostat sections from colon adenocarcinoma by the indirect immunoperoxidase technique. The specific reaction was compared with that obtained by using a similar technique and two CEA specific monoclonal antibodies. An anti-CEA IgG peroxidase conjugate was obtained allowing to establish a "sandwich" ELISA-CEA system with two antibodies. CEA determinations were made in a group of 15 normal controls (mean value 4.8 +/- 0.4 ng/ml) and in 30 colorectal tumor patients (mean value 26.6 +/- 2.15 ng/ml). The anti-CEA antibodies are proven useful in immunocytochemical and ELISA techniques and may be further used in radioimaging of tumors.
利用从人结肠腺癌酸性提取物中纯化得到的癌胚抗原（CEA），制备了羊和兔的抗CEA多克隆抗体。CEA通过Sepharose 4B CL凝胶过滤和DEAE-Sephadex A50柱层析进行纯化。抗血清用正常人血清和正常结肠的高氯酸提取物进行吸附。通过离子交换层析从单特异性抗血清中纯化抗CEA IgG，并通过间接免疫过氧化物酶技术在结肠腺癌冰冻切片上检测其特异性。将该特异性反应与使用类似技术和两种CEA特异性单克隆抗体所获得的反应进行比较。获得了抗CEA IgG过氧化物酶偶联物，从而建立了一种使用两种抗体的“夹心”ELISA-CEA系统。对15名正常对照者（平均值4.8 +/- 0.4 ng/ml）和30名结直肠肿瘤患者（平均值26.6 +/- 2.15 ng/ml）进行了CEA测定。抗CEA抗体在免疫细胞化学和ELISA技术中已被证明是有用的，并且可能进一步用于肿瘤的放射成像。
