Sun Jia, Yu Jin, Zhang Pei-Cheng, Yue Yong-De
State Forestry Administration Key Open Laboratory, International Centre for Bamboo and Rattan, Beijing, 100102, China.
Phytochem Anal. 2015 Jan-Feb;26(1):54-60. doi: 10.1002/pca.2536. Epub 2014 Sep 4.
Bambusa tuldoides Munro, a bamboo species, is used as a health food, dietary supplement and folk medicine in China, and produces lignans that can be used to supplement other natural sources.
To simultaneously separate eight stereoisomers of a particular type of oxyneolignan by chiral chromatography.
Ninety-five per cent ethanol extracts of B. tuldoides Munro were analysed using HPLC/UV with a chiral column. The structures and configurations of isolated compounds were elucidated using NMR and circular dichroism (CD).
Four diastereoisomers were characterised and given the names oxyneolignans A, B, C and D. Furthermore, each oxyneolignan occurred as a pair of enantiomers. The oxyneolignans A-D consisted of the erythro-diastereoisomer of oxyneolignan at C7 and C8.
The chiral chromatography combined with the analysis techniques of NMR and CD reported here were reliable methods for discovering and separating the enantiomers.
绿竹（Bambusa tuldoides Munro）是一种竹子，在中国被用作保健食品、膳食补充剂和民间药物，并且能产生可用于补充其他天然来源的木脂素。
通过手性色谱法同时分离一种特定类型的氧化新木脂素的八种立体异构体。
采用高效液相色谱/紫外检测法，使用手性柱对绿竹的95%乙醇提取物进行分析。利用核磁共振（NMR）和圆二色光谱（CD）对分离得到的化合物的结构和构型进行鉴定。
鉴定出四种非对映异构体，并将其命名为氧化新木脂素A、B、C和D。此外，每种氧化新木脂素均以一对对映体的形式存在。氧化新木脂素A - D由C7和C8位氧化新木脂素的赤藓型非对映异构体组成。
本文报道的手性色谱法与核磁共振和圆二色光谱分析技术相结合是发现和分离对映体的可靠方法。
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