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脊髓内移植运动神经元样细胞联合聚合物载体胶质细胞源性神经营养因子递送来修复脊髓挫裂伤。

Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor for repair of spinal cord contusion injury.

机构信息

Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, Iran.

出版信息

Neural Regen Res. 2014 May 15;9(10):1003-13. doi: 10.4103/1673-5374.133159.

DOI:10.4103/1673-5374.133159
PMID:25206752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4146307/
Abstract

To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord contusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact site and simultaneously transplanted glial cell line-derived neurotrophic factor-gelfoam complex into the myelin sheath. Motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery reduced cavity formations and increased cell density in the transplantation site. The combined therapy exhibited superior promoting effects on recovery of motor function to transplantation of glial cell line-derived neurotrophic factor, adipose-derived stem cells or motoneurons alone. These findings suggest that motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery holds a great promise for repair of spinal cord injury.

摘要

为了评估胶质细胞源性神经营养因子移植联合脂肪来源干细胞诱导分化的运动神经元移植对脊髓挫伤损伤的影响，我们建立了大鼠脊髓挫伤损伤模型，7 天后，将脂肪来源干细胞诱导分化的运动神经元注射到撞击部位的中心、头侧和尾侧区域，并同时将胶质细胞源性神经营养因子-明胶海绵复合物移植到髓鞘中。运动神经元样细胞移植联合胶质细胞源性神经营养因子递呈减少了腔形成并增加了移植部位的细胞密度。与单独移植胶质细胞源性神经营养因子、脂肪来源干细胞或运动神经元相比，联合治疗对运动功能恢复具有更好的促进作用。这些发现表明，运动神经元样细胞移植联合胶质细胞源性神经营养因子递呈为脊髓损伤修复带来了很大的希望。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/16680ea58c21/NRR-9-1003-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/d71529030974/NRR-9-1003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/b89985c980a6/NRR-9-1003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/cd0dd3ab072b/NRR-9-1003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/f7682558650b/NRR-9-1003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/0d2fddf93763/NRR-9-1003-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/16680ea58c21/NRR-9-1003-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/d71529030974/NRR-9-1003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/b89985c980a6/NRR-9-1003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/cd0dd3ab072b/NRR-9-1003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/f7682558650b/NRR-9-1003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/0d2fddf93763/NRR-9-1003-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1312/4146307/16680ea58c21/NRR-9-1003-g007.jpg

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