Papanikolaou Nikolaos A, Tillinger Andrej, Liu Xiaoping, Papavassiliou Athanasios G, Sabban Esther L
Laboratory of Biological Chemistry, Department of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Hellas (Greece).
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, 10595, USA.
BMC Syst Biol. 2014 Sep 11;8:100. doi: 10.1186/s12918-014-0100-8.
Adaptation to stress is critical for survival. The adrenal medulla, the major source of epinephrine, plays an important role in the development of the hyperadenergic state and increased risk for stress associated disorders, such as hypertension and myocardial infarction. The transcription factor Egr1 plays a central role in acute and repeated stress, however the complexity of the response suggests that other transcription factor pathways might be playing equally important roles during acute and repeated stress. Therefore, we sought to discover such factors by applying a systems approach.
Using microarrays and network analysis we show here for the first time that the transcription factor signal transducer and activator of transcription 3 (Stat3) gene is activated in acute stress whereas the prolactin releasing hormone (Prlh11) and chromogranin B (Chgb) genes are induced in repeated immobilization stress and that along with Egr1 may be critical mediators of the stress response.
Our results suggest possible involvement of Stat3 and Prlh1/Chgb up-regulation in the transition from short to repeated stress activation.
适应压力对生存至关重要。肾上腺髓质是肾上腺素的主要来源，在高肾上腺素状态的发展以及与压力相关疾病（如高血压和心肌梗死）风险增加方面发挥重要作用。转录因子Egr1在急性和反复应激中起核心作用，然而反应的复杂性表明其他转录因子途径在急性和反复应激期间可能发挥同样重要的作用。因此，我们试图通过应用系统方法来发现此类因素。
利用微阵列和网络分析，我们首次在此表明转录因子信号转导和转录激活因子3（Stat3）基因在急性应激中被激活，而催乳素释放激素（Prlh11）和嗜铬粒蛋白B（Chgb）基因在反复固定应激中被诱导，并且与Egr1一起可能是应激反应的关键介质。
我们的结果表明Stat3以及Prlh1/Chgb上调可能参与从短期应激激活到反复应激激活的转变。
