Zheng Yujia, Yang Zhongyi, Zhang Yongping, Shi Qi, Bao Xiao, Zhang Jianping, Yuan Huiyu, Yao Zhifeng, Hu Chaosu, Zhang Yingjian
Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, No. 270, Dong'an Road, Xuhui District, Shanghai, China.
Ann Nucl Med. 2015 Jan;29(1):29-36. doi: 10.1007/s12149-014-0906-x. Epub 2014 Sep 13.
The purpose of the preliminary study was to investigate the value of (18)F-FLT micro-PET/CT in predicting radiosensitivity of human nasopharyngeal carcinoma (NPC) xenografts in nude mice models.
Twelve BALB/c-nu nude mice were randomly divided into two groups. They were subcutaneously injected with either CNE1 or CNE2 cell suspension. Xenograft volumes were measured after tumor formation. When the tumors reached nearly 10 mm in diameter, they received 15-Gy irradiation. Before and 24 h after irradiation, mice were performed with (18)F-FLT micro-PET/CT. The region of interest (ROI) was manually drawn, and the percent of injected dose per gram of the tumor and muscle in the ROIs was recorded. Tumor-to-muscle ratio (T/M) was calculated and compared with volume changes. Additionally, we also used ten untreated mice as control group.
After irradiation, CNE2 tumors decreased significantly while CNE1 tumors continuously grew and became stable after 1 week. However, in control group, CNE1 and CNE2 tumors continuously enlarged in the observed time. Therefore, we could regard CNE2 group as irradiation responder while CNE1 group as non-responder. In irradiation group, the value of T/M before irradiation (T/M 0) of CNE1 mice was statistically lower than CNE2 mice (1.62 ± 0.38 versus 5.57 ± 1.30; P = 0.004). Besides, T/M decreased significantly in CNE2 group after irradiation (5.57 ± 1.30 versus 3.59 ± 1.06; P < 0.001). By means of a receiver operating characteristic curve, the optimal cut value of T/M 0 and ∆T/M to predict responder was 2.38 and -0.15, respectively (both sensitivity and specificity = 100.0 %).
(18)F-FLT PET/CT has the potential to predict radiosensitivity in NPC xenografts nude mice models.
本初步研究的目的是探讨¹⁸F - FLT微型正电子发射断层显像/计算机断层扫描（¹⁸F - FLT micro - PET/CT）在预测裸鼠模型中人鼻咽癌（NPC）移植瘤放射敏感性方面的价值。
将12只BALB/c - nu裸鼠随机分为两组。分别皮下注射CNE1或CNE2细胞悬液。肿瘤形成后测量移植瘤体积。当肿瘤直径达到近10毫米时，给予15 Gy的照射。在照射前和照射后24小时，对小鼠进行¹⁸F - FLT micro - PET/CT检查。手动绘制感兴趣区（ROI），记录ROI中每克肿瘤和肌肉的注射剂量百分比。计算肿瘤与肌肉比值（T/M）并与体积变化进行比较。此外，我们还将10只未治疗的小鼠作为对照组。
照射后，CNE2肿瘤显著缩小，而CNE1肿瘤持续生长并在1周后稳定。然而，在对照组中，CNE1和CNE2肿瘤在观察期内持续增大。因此，我们可将CNE2组视为照射反应组，而CNE1组视为无反应组。在照射组中，CNE1小鼠照射前的T/M值（T/M 0）在统计学上低于CNE2小鼠（1.62±0.38对5.57±1.30；P = 0.004）。此外，照射后CNE2组的T/M显著降低（5.57±1.30对3.59±1.06；P < 0.001）。通过受试者工作特征曲线，预测反应组的T/M 0和∆T/M的最佳截断值分别为2.38和 - 0.15（敏感性和特异性均为100.0%）。
¹⁸F - FLT PET/CT有潜力预测NPC移植瘤裸鼠模型的放射敏感性。
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