一种针对脑膜炎奈瑟菌X群荚膜多糖的新型单克隆抗体及其在脑膜炎球菌疫苗定量中的潜在应用。
A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines.
作者信息
Reyes Fátima, Otero Oscar, Camacho Frank, Amin Nevis, Ramírez Fidel, Valdés Yolanda, Acevedo Reynaldo, García Luis, Cardoso Daniel, Cuello Maribel
机构信息
Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba; Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Concepción, P.O. Box 160C, Chile.
Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
出版信息
Biologicals. 2014 Nov;42(6):312-5. doi: 10.1016/j.biologicals.2014.08.001. Epub 2014 Sep 11.
A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 10(7) M(-1). The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.
为了开发一种用于定量脑膜炎球菌多糖的夹心酶联免疫吸附测定（ELISA），制备了一种针对X群脑膜炎奈瑟菌（MenX）荚膜多糖（CP）的新型鼠杂交瘤单克隆抗体（MAb）。该单克隆抗体仅与MenX的CP反应，而不与A、C、Y和W群脑膜炎奈瑟菌（MenA、MenC、MenY、MenW）的CP反应。通过非竞争性ELISA测定的该单克隆抗体的亲和常数（Ka）为7.25×10⁷ M⁻¹。该单克隆抗体在夹心ELISA中的应用通过其正确定量三批基于实验性脑膜炎球菌CP的疫苗的能力得到证明。在这项工作中获得的单克隆抗体可能是检测和定量未来含MenX CP的脑膜炎球菌疫苗的有价值试剂。
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