Sina Abu Ali Ibn, Howell Sidney, Carrascosa Laura G, Rauf Sakandar, Shiddiky Muhammad J A, Trau Matt
Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia.
Chem Commun (Camb). 2014 Nov 7;50(86):13153-6. doi: 10.1039/c4cc06732k.
We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
我们报告了一种用于检测DNA甲基化的简单电化学方法，称为“eMethylsorb”。该方法依赖于DNA与金之间基于碱基的亲和相互作用。通过监测电化学电流作为亚硫酸氢盐处理的DNA样品在裸金电极上相对吸附水平的函数，对DNA的甲基化状态进行定量。该方法能够在单CpG分辨率下成功区分甲基化和未甲基化的表观基因型。
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