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T 细胞激活。I. 关于 I 类主要组织相容性复合体抗原与细胞毒性 T 淋巴细胞 -T 细胞受体复合物之间功能联系的证据。

T-cell activation. I. Evidence for a functional linkage between class I MHC antigens and the Tc-Ti complex.

作者信息

Brams P, Claesson M H

机构信息

Receptron, Concord, California.

出版信息

Immunology. 1989 Mar;66(3):348-53.

PMID:2522906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1385219/
Abstract

This paper examines the possibility of a functional linkage between class I MHC molecules and the T-cell receptor complex for antigen (T3-Ti). A newly developed anti-CD3 antibody (500A2) was used as an activation signal for EL4 lymphoma cells and allospecific cytotoxic T-cell clones (CTL), and the production of IL-2/IL-2 receptor in EL4 cells and serine esterase in CTL was determined. Anti-CD3 antibody-induced activation of both EL4 and CTL cells was enhanced in the presence of immunologically cross-linked and immobilized anti-H-2 (class I) antibody reactive against the H-2 haplotype of the responding T cells. A number of H-2-negative and H-2-positive EL4 subclones were generated and tested for anti-CD3 antibody-induced IL-2/IL-2 receptor production. Although both H-2-positive and -negative subclones expressed CD3 antigen and produced IL-2 after activation with the phorbol ester TPA, only the H-2-positive cell clones produced IL-2 and expressed IL-2 receptor after anti-CD3 antibody induction. Our results are compatible with the existence of a functional linkage between the class I and the CD3 molecules on the surface of T cells.

摘要

本文研究了I类主要组织相容性复合体（MHC）分子与抗原特异性T细胞受体复合物（T3-Ti）之间功能联系的可能性。一种新开发的抗CD3抗体（500A2）被用作EL4淋巴瘤细胞和同种异体特异性细胞毒性T细胞克隆（CTL）的激活信号，并测定了EL4细胞中白细胞介素-2（IL-2）/IL-2受体的产生以及CTL中丝氨酸酯酶的产生。在存在与应答T细胞的H-2单倍型发生免疫交联并固定的抗H-2（I类）抗体的情况下，抗CD3抗体诱导的EL4细胞和CTL细胞的激活增强。产生了许多H-2阴性和H-2阳性的EL4亚克隆，并检测了抗CD3抗体诱导的IL-2/IL-2受体产生情况。尽管H-2阳性和阴性亚克隆在用佛波酯TPA激活后均表达CD3抗原并产生IL-2，但只有H-2阳性细胞克隆在抗CD3抗体诱导后产生IL-2并表达IL-2受体。我们的结果与T细胞表面I类分子和CD3分子之间存在功能联系的观点相符。

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引用本文的文献

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Activation of lymphocytes after platelet allotransfusion possessing only class I MHC product.仅具有I类主要组织相容性复合体产物的血小板异体输血后淋巴细胞的激活。
Clin Exp Immunol. 1990 Oct;82(1):102-7. doi: 10.1111/j.1365-2249.1990.tb05411.x.

本文引用的文献

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Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer.通过DNA介导的基因转移鉴定BALB/c H-2Ld基因。
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Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines.用于细胞活力的快速比色测定法：应用于细胞毒性和生长抑制性淋巴因子的定量分析。
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Human and murine class I MHC antigens share conserved serine 335, the site of HLA phosphorylation in vivo.人类和鼠类的I类主要组织相容性复合体（MHC）抗原共有保守的丝氨酸335，这是体内HLA磷酸化的位点。
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Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway.T3-Ti抗原受体复合物的触发通过白细胞介素2依赖的自分泌途径导致克隆性T细胞增殖。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1509-13. doi: 10.1073/pnas.81.5.1509.
6
Differential effect of anti-beta 2-microglobulin on IL 2 production and IL 2 receptor expression in the primary mixed lymphocyte culture reaction.抗β2-微球蛋白对原发性混合淋巴细胞培养反应中白细胞介素2产生及白细胞介素2受体表达的差异作用。
J Immunol. 1985 Feb;134(2):940-8.
7
Phosphorylation of the CD8 antigen on cytotoxic human T cells in response to phorbol myristate acetate or antigen-presenting B cells.细胞毒性人T细胞上CD8抗原的磷酸化反应，该反应由佛波酯肉豆蔻酸酯乙酸盐或抗原呈递B细胞引发。
J Immunol. 1987 Oct 1;139(7):2268-74.
8
The role of class I histocompatibility antigens in the regulation of T-cell activation.I类组织相容性抗原在T细胞活化调节中的作用。
Proc Natl Acad Sci U S A. 1987 Feb;84(4):1094-8. doi: 10.1073/pnas.84.4.1094.
9
Synergism in the activation of human CD8 T cells by cross-linking the T-cell receptor complex with the CD8 differentiation antigen.通过将T细胞受体复合物与CD8分化抗原交联来激活人CD8 T细胞中的协同作用。
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8298-302. doi: 10.1073/pnas.83.21.8298.
10
The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens.作为人类细胞膜胰岛素受体结构亚基的主要组织相容性复合体I类重链：对组织相容性抗原生物学功能范围的影响。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6007-11. doi: 10.1073/pnas.83.16.6007.