Perry R R, Evans S, Matthews W, Rizzoni W, Russo A, Pass H I
Thoracic Oncology Section, National Cancer Institute, Bethesda, Maryland 20892.
J Surg Res. 1989 Apr;46(4):386-90. doi: 10.1016/0022-4804(89)90207-2.
Lung cancer cells are susceptible to photodynamic therapy (PDT) using 630 nm light and dihematoporphyrin ether (DHE). A light scattering media, intralipid (IL), was compared to balanced salt solution (PBS) for PDT of A549 human lung cancer cells. Differences in cellular DHE content after IL or PBS exposure were determined. Cells were incubated in 25 micrograms/ml DHE for 2 hr and then incubated in various concentrations of IL or PBS at room temperature for 2.5 to 10.0 min. Significant amounts of DHE were lost from IL-incubated cells compared to cells incubated in PBS. After 5 min in 1% IL, cellular DHE content was 0.32 +/- 0.04 microgram DHE/10(6) cells compared to 0.56 +/- 0.11 microgram DHE/10(6) cells in PBS-incubated cells (P less than 0.05). Despite this, superior PDT cytotoxicity was noted when cells were treated in IL with energy densities greater than or equal to 105 mJ/cm2. At an energy density of 210 mJ/cm2, the survival fraction (SF) of cells treated in 1% IL was 0.004 +/- 0.001 compared to 0.071 +/- 0.022 in PBS-treated cells (P less than 0.05). SF was dependent upon the IL concentration with the greatest cell killing noted with 1% IL. An apparent loss of cellular DHE ("DHE washout") was confirmed by demonstration of a higher SF of cells incubated in IL, rinsed, and subsequently PDT-treated in PBS with 157.5 mJ/cm2 (SF = 0.85 +/- 0.11) compared to cells incubated and treated in PBS (SF = 0.50 +/- 0.03, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
肺癌细胞对使用630纳米光和二血卟啉醚（DHE）的光动力疗法（PDT）敏感。将一种光散射介质——脂悬液（IL）与平衡盐溶液（PBS）相比较，用于对A549人肺癌细胞进行光动力疗法。测定了IL或PBS处理后细胞内DHE含量的差异。细胞在25微克/毫升DHE中孵育2小时，然后在室温下于不同浓度的IL或PBS中孵育2.5至10.0分钟。与在PBS中孵育的细胞相比，在IL中孵育的细胞损失了大量DHE。在1%IL中孵育5分钟后，细胞内DHE含量为0.32±0.04微克DHE/10⁶个细胞，而在PBS中孵育的细胞为0.56±0.11微克DHE/10⁶个细胞（P<0.05）。尽管如此，当细胞在IL中接受能量密度大于或等于105毫焦/平方厘米的处理时，观察到了更好的光动力疗法细胞毒性。在能量密度为210毫焦/平方厘米时，在1%IL中处理的细胞存活分数（SF）为0.004±0.001，而在PBS处理的细胞中为0.071±0.022（P<0.05）。存活分数取决于IL浓度，1%IL时细胞杀伤作用最大。通过证明在IL中孵育、冲洗，随后在PBS中用157.5毫焦/平方厘米进行光动力疗法处理的细胞（存活分数=0.85±0.11）比在PBS中孵育和处理的细胞（存活分数=0.50±0.03，P<0.05）具有更高的存活分数，证实了细胞内DHE存在明显损失（“DHE洗脱”）。（摘要截断于250字）
