毛细管电泳法对橄榄油中辛辣和抗炎酚类化合物的定量分析
Quantitative analysis of pungent and anti-inflammatory phenolic compounds in olive oil by capillary electrophoresis.
作者信息
Vulcano Isabella, Halabalaki Maria, Skaltsounis Leandros, Ganzera Markus
机构信息
Institute of Pharmacy, Pharmacognosy, Member of CMBI, University of Innsbruck, 6020 Innsbruck, Austria.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens 15771, Greece.
出版信息
Food Chem. 2015 Feb 15;169:381-6. doi: 10.1016/j.foodchem.2014.08.007. Epub 2014 Aug 11.
The first CE procedure for the quantitative determination of pharmacologically relevant secoiridoids in olive oil, oleocanthal and oleacein, is described. Together with their precursors tyrosol and hydroxytyrosol they could be baseline separated in less than 15min using a borax buffer with pH 9.5, at 25kV and 30°C. Method validation confirmed that the procedure is selective, accurate (recovery rates from 94.0 to 104.6%), reproducible (σmax⩽6.8%) and precise (inter-day precision⩽6.4%), and that the compounds do not degrade quickly if non-aqueous acetonitrile is used as solvent. Quantitative results indicated a low occurrence of oleocanthal (0.004-0.021%) and oleacein (0.002-0.048%) in olive oil samples, which is in agreement to published HPLC data. The CE method impresses with its simple instrumental and methodological design, combined with reproducible and valid quantitative results.
本文描述了首个用于定量测定橄榄油中具有药理活性的裂环烯醚萜类化合物油橄榄苦素和橄榄苦苷的毛细管电泳（CE）方法。使用pH 9.5的硼砂缓冲液，在25 kV和30°C条件下，它们与前体酪醇和羟基酪醇能够在不到15分钟的时间内实现基线分离。方法验证表明，该方法具有选择性、准确性（回收率为94.0%至104.6%）、可重复性（σmax⩽6.8%）和精密度（日间精密度⩽6.4%），并且如果使用非水乙腈作为溶剂，这些化合物不会快速降解。定量结果表明，橄榄油样品中油橄榄苦素（0.004 - 0.021%）和橄榄苦苷（0.002 - 0.048%）的含量较低，这与已发表的高效液相色谱（HPLC）数据一致。CE方法以其简单的仪器和方法设计以及可重复且有效的定量结果给人留下深刻印象。
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