Ma Qingzhan, Wu Bo, Li Jiuxuan, Song Zhaobin
a Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Chengdu , China and.
b Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1683-4. doi: 10.3109/19401736.2014.958727. Epub 2014 Sep 19.
The complete mitochondrial DNA genome of Ptychobarbus kaznakovi was sequenced and characterized. The genome is 16,842 bp in length. Similar with most teleosts, it has two ribosomal RNA (rRNA) genes, 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and one displacement loop (D-loop) region. Conserved sequence blocks, including ETAS, CSB-B, D, E, F, and CSB1-3, were identified in the D-loop, which is similar to other species in Cypriniformes. Nevertheless, a 55 bp tandem repeat array was also identified at 3' end of the D-loop, which is the first finding in Schizothoracinae. Phylogenetic analysis showed that the species of Ptychobarbus (P. dipogon and P. kaznakovi) formed a monophyletic group and represented close relationship to the species without scales in Schizothoracinae.
对高原裸鲤的完整线粒体DNA基因组进行了测序和特征分析。该基因组长度为16,842 bp。与大多数硬骨鱼相似,它有两个核糖体RNA(rRNA)基因、13个蛋白质编码基因、22个转运RNA(tRNA)基因和一个位移环(D-loop)区域。在D-loop中鉴定出了保守序列块,包括ETAS、CSB-B、D、E、F和CSB1-3,这与鲤形目其他物种相似。然而,在D-loop的3'端还鉴定出了一个55 bp的串联重复序列阵列,这是裂腹鱼亚科中的首次发现。系统发育分析表明,裸鲤属物种(双须叶须鱼和高原裸鲤)形成了一个单系群,并且与裂腹鱼亚科中无鳞的物种关系密切。