Eryürük Kağan, Yang Suyin, Suzuki Daisuke, Sakaguchi Iwao, Akatsuka Tetsuji, Tsuchiya Takayuki, Katayama Arata
Graduate School of Engineering, Department of Civil Engineering, Nagoya University, Nagoya 464-8601, Japan.
EcoTopia Science Institute, Division of Environmental Research, Nagoya University, Nagoya 464-8603, Japan.
J Biosci Bioeng. 2015 Mar;119(3):331-6. doi: 10.1016/j.jbiosc.2014.08.009. Epub 2014 Sep 16.
The effect on hydraulic conductivity in porous media of CaCO3 precipitation induced by Sporosarcina pasteurii (ATCC 11859) was investigated using continuous-flow columns containing glass beads between 0.01 mm and 3 mm in diameter. Resting S. pasteurii cells and a precipitation solution composed of 0.5 M CaCl2 and 0.5 M urea were introduced into the columns, and it was shown that the subsequent formation of CaCO3 precipitation reduced hydraulic conductivity from between 8.38 × 10(-1) and 3.27 × 10(-4) cm/s to between 3.70 × 10(-1) and 3.07 × 10(-5) cm/s. The bacterial cells themselves did not decrease the hydraulic conductivity. The amount of precipitation was proportional with the bacterial number in the column. The specific CaCO3 precipitation rate of the resting cells was estimated as 4.0 ± 0.1 × 10(-3) μg CaCO3/cell. Larger amounts of CaCO3 precipitation were deposited in columns packed with small glass beads than in those packed with large glass beads, resulting in a greater reduction in the hydraulic conductivity of the columns containing small glass beads. Analysis using the Kozeny-Carman equation suggested that the effect of microbially induced CaCO3 precipitation on hydraulic conductivity was not due to the formation of individual CaCO3 crystals but instead that the precipitate aggregated with the glass beads, thus increasing their diameter and consequently decreasing the pore size in the column.
利用装有直径在0.01毫米至3毫米之间玻璃珠的连续流动柱，研究了巴氏芽孢杆菌（ATCC 11859）诱导的碳酸钙沉淀对多孔介质水力传导率的影响。将静止的巴氏芽孢杆菌细胞以及由0.5 M氯化钙和0.5 M尿素组成的沉淀溶液引入柱中，结果表明，随后形成的碳酸钙沉淀使水力传导率从8.38×10⁻¹至3.27×10⁻⁴厘米/秒降低至3.70×10⁻¹至3.07×10⁻⁵厘米/秒。细菌细胞本身并未降低水力传导率。沉淀量与柱中的细菌数量成正比。静止细胞的碳酸钙比沉淀速率估计为4.0±0.1×10⁻³微克碳酸钙/细胞。与装有大玻璃珠的柱相比，装有小玻璃珠的柱中沉积了更多的碳酸钙沉淀，导致装有小玻璃珠的柱的水力传导率降低幅度更大。使用柯曾尼-卡曼方程进行的分析表明，微生物诱导的碳酸钙沉淀对水力传导率的影响并非由于单个碳酸钙晶体的形成，而是沉淀与玻璃珠聚集在一起，从而增加了它们的直径，进而减小了柱中的孔径。
