高山被孢霉CBS 754.68中花生四烯酸生物合成相关基因的表达分析
Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68.
作者信息
Samadlouie Hamid-Reza, Hamidi-Esfahani Zohreh, Alavi Seyed-Mehdi, Varastegani Boshra
机构信息
Department of Food Science and Technology Faculty of Agriculture Tarbiat Modares University Tehran Iran Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
National institute for Genetic Engineering and Biotechnology Tehran Iran National institute for Genetic Engineering and Biotechnology, Tehran, Iran.
出版信息
Braz J Microbiol. 2014 Aug 29;45(2):439-45. doi: 10.1590/s1517-83822014000200010. eCollection 2014.
The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.
在高山被孢霉CBS 754.68中检测了真菌生物量、脂质、酚类和花生四烯酸（ARA）的产生时间进程以及参与ARA和脂质生物合成的基因表达。脂质中花生四烯酸含量显著增加，同时脂质水平降低。基因表达降低可能是由于碳和氮资源的持续减少。然而，这些能源未被积累脂质的分解有效补偿，这反过来诱导了候选基因的上调表达。结果进一步表明，编码GLELO的基因的表达是早期生长阶段ARA生物合成中的限速步骤。
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