运用相关光镜和电子显微镜技术研究斑马鱼血管形态发生。

Using correlative light and electron microscopy to study zebrafish vascular morphogenesis.

作者信息

Goetz Jacky G, Monduc Fabien, Schwab Yannick, Vermot Julien

机构信息

The Microenvironmental Niche in Tumorigenesis and Targeted Therapy, Inserm U1109, LabEx Medalis, Fédération de Médecine Translationnelle de Strasbourg (FMTS), 67000, Strasbourg, France.

出版信息

Methods Mol Biol. 2015;1189:31-46. doi: 10.1007/978-1-4939-1164-6_3.

DOI:10.1007/978-1-4939-1164-6_3

PMID:25245685

Abstract

Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.

摘要

实时成像对于在体内表征细胞事件的动态极为有用，但在空间分辨率方面存在局限性。 correlative light and electron microscopy (CLEM) 允许将实时共聚焦显微镜与电子显微镜 (EM) 相结合，以在高时间和空间分辨率下表征生物样品。在这里，我们描述了一种方案，该方案允许在斑马鱼胚胎发育过程中通过实时共聚焦成像在体内对同一细胞进行成像后提取内皮细胞超微结构。