Yang Xiaoming, Feng Yuanjiao, Zhu Shanshan, Luo Yawen, Zhuo Yan, Dou Yao
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Anal Chim Acta. 2014 Oct 17;847:49-54. doi: 10.1016/j.aca.2014.07.019. Epub 2014 Jul 19.
Herein, an innovative and simple strategy for synthesizing high fluorescent Cu nanoclusters was successfully established while L-cysteine played a role as the stabilizer. Meaningfully, the current Cu nanoclusters together with a quantum yield of 14.3% were prepared in aqueous solution, indicating their extensive applications. Subsequently, the possible fluorescence mechanism was elucidated by fluorescence, UV-vis, HR-TEM, FTIR, XPS, and MS. Additionally, the CuNCs were employed for assaying Hg(2+) on the basis of the interactions between Hg(2+) and L-cysteine; thus facilitating the quenching of their fluorescence. The proposed analytical strategy permitted detections of Hg(2+) in a linear range of 1.0×10(-7) mol L(-1)×10(-3) mol L(-1), with a detection limit of 2.4×10(-8) mol L(-1) at a signal-to-noise ratio of 3. Significantly, this CuNCs described here were further applied for coding and fluorescent staining, suggesting may broaden avenues toward diverse applications.
在此，成功建立了一种创新且简单的合成高荧光铜纳米簇的策略，其中L-半胱氨酸作为稳定剂。有意义的是，当前的铜纳米簇在水溶液中制备，量子产率为14.3%，表明它们具有广泛的应用。随后，通过荧光、紫外可见光谱、高分辨透射电子显微镜、傅里叶变换红外光谱、X射线光电子能谱和质谱阐明了可能的荧光机制。此外，基于Hg(2+)与L-半胱氨酸之间的相互作用，铜纳米簇被用于检测Hg(2+)；从而促进其荧光猝灭。所提出的分析策略允许在1.0×10(-7) mol L(-1)至10(-3) mol L(-1)的线性范围内检测Hg(2+)，在信噪比为3时检测限为2.4×10(-8) mol L(-1)。值得注意的是，这里描述的这种铜纳米簇进一步应用于编码和荧光染色，表明可能会拓宽通往多种应用的途径。
