Hill Robert A, Patel Kiran D, Goncalves Christopher M, Grutzendler Jaime, Nishiyama Akiko
1] Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA. [2] Department of Neurology, Yale University School of Medicine, New Haven, Connecticut, USA.
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA.
Nat Neurosci. 2014 Nov;17(11):1518-27. doi: 10.1038/nn.3815. Epub 2014 Sep 28.
Oligodendrocytes in the mammalian brain are continuously generated from NG2 cells throughout postnatal life. However, it is unclear when the decision is made for NG2 cells to self-renew or differentiate into oligodendrocytes after cell division. Using a combination of in vivo and ex vivo imaging and fate analysis of proliferated NG2 cells in fixed tissue, we demonstrate that in the postnatal developing mouse brain, the majority of divided NG2 cells differentiate into oligodendrocytes during a critical age-specific temporal window of 3-8 d. Notably, within this time period, damage to myelin and oligodendrocytes accelerated oligodendrocyte differentiation from divided cells, and whisker removal decreased the survival of divided cells in the deprived somatosensory cortex. These findings indicate that during the critical temporal window of plasticity, the fate of divided NG2 cells is sensitive to modulation by external signals.
在哺乳动物大脑中，少突胶质细胞在出生后的整个生命过程中持续由NG2细胞产生。然而，目前尚不清楚NG2细胞在细胞分裂后决定自我更新或分化为少突胶质细胞的时间点。通过结合体内和体外成像以及对固定组织中增殖的NG2细胞进行命运分析，我们证明在出生后发育的小鼠大脑中，大多数分裂的NG2细胞在3至8天这个关键的年龄特异性时间窗口内分化为少突胶质细胞。值得注意的是，在此时间段内，髓鞘和少突胶质细胞的损伤加速了分裂细胞向少突胶质细胞的分化，而去除触须则降低了躯体感觉皮层被剥夺区域中分裂细胞的存活率。这些发现表明，在可塑性的关键时间窗口期间，分裂的NG2细胞的命运对外界信号的调节敏感。
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