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细胞角蛋白、N-钙黏蛋白和CD133在转移性乳腺癌患者循环肿瘤细胞中的差异表达。

Distinct expression of cytokeratin, N-cadherin and CD133 in circulating tumor cells of metastatic breast cancer patients.

作者信息

Bock Carolin, Rack Brigitte, Huober Jens, Andergassen Ulrich, Jeschke Udo, Doisneau-Sixou Sophie

机构信息

Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Campus Innenstadt, Klinikum der Ludwig-Maximilians-Universität, Maistraße 11, 80337 Munich, Germany.

出版信息

Future Oncol. 2014 Aug;10(10):1751-65. doi: 10.2217/fon.14.58.

DOI:10.2217/fon.14.58

PMID:25303055

Abstract

AIM

Circulating tumor cells (CTCs) appear as potential candidates to predict the ability of breast tumors to metastasize. Moreover, epithelial-mesenchymal transition (EMT) and stem cell features are major mechanisms for metastasis.

PATIENTS & METHODS: Using a triple fluorescence technique, the expression of EMT (N-cadherin) and stem cell markers (CD133) was analyzed in CTCs detected via cytokeratin in blood samples from 26 metastatic breast cancer patients.

RESULTS

We detected CTCs in 100% of the patients (n = 831 CTCs). In total, 67% of the CTCs were N-cadherin and CD133 negative. Nonetheless, 87.8 and 57.6%, respectively, of the CTCs that expressed one marker coexpressed the other. Both double-negative and double-positive CTCs were present in more than 90% of the patients. Within the CTCs of each patient, we demonstrated striking heterogeneities of marker expressions, cell shapes, clusters and sizes.

CONCLUSION

These data outline the importance of characterizing CTCs, especially through stem cell and EMT markers.

摘要

目的

循环肿瘤细胞（CTCs）似乎是预测乳腺肿瘤转移能力的潜在候选者。此外，上皮-间质转化（EMT）和干细胞特征是转移的主要机制。

患者与方法

采用三重荧光技术，对26例转移性乳腺癌患者血样中通过细胞角蛋白检测到的CTCs中EMT（N-钙黏蛋白）和干细胞标志物（CD133）的表达进行分析。

结果

我们在所有患者（共831个CTCs）中均检测到了CTCs。总体而言，67%的CTCs为N-钙黏蛋白和CD133阴性。然而，分别有87.8%和57.6%表达一种标志物的CTCs同时表达另一种标志物。双阴性和双阳性CTCs在90%以上的患者中均有存在。在每位患者的CTCs中，我们证实了标志物表达、细胞形状、细胞簇和大小存在显著异质性。