Department of Clinical Epidemiology, Predictive Medicine and Public Health, University of Porto Medical School, Porto, Portugal - Department of Science, Oral Microbiology Laboratory, Instituto Superior de Ciências da Saúde-Norte, Gandra, Portugal.
Department of Science, Oral Microbiology Laboratory, Instituto Politécnico de Ciências da Saúde-Norte, Gandra, Portugal.
Eur J Paediatr Dent. 2014 Sep;15(3):281-7.
To evaluate the association between dental health status and levels of cariogenic bacteria in teenagers and the influence of behaviours and socio-demographic background on levels of bacteria.
Study design: A cross-sectional population-based sample of 13-year-old adolescents (112 females and 78 males, total 190) was examined. The number of decayed, missing and filled surfaces (DMFS), plaque and hygiene index were recorded according to the WHO criteria. The saliva samples were collected in a sterile container and then analysed by culture on Mitis Salivarius Bacitracin (MSB) agar for mutans Streptococci and on Man Rogosa Sharp (MRS) agar for Lactobacilli. The levels of bacteria were expressed as the number of colonies forming units per millilitre of saliva (CFU/ml). Associations between levels of mutans Streptococci and Lactobacilli and dental health were estimated by odds ratio (OR) and 95% confidence interval (95% CI) using unconditional logistic regression.
No mutans Streptococci were detected in 53.2% of the adolescents but 22.6% presented ≥ 10³ CFU/ml. For Lactobacilli, these values were, respectively 43.7% and 34.7%. After adjustment for gender and social class, the OR (95% CI) for DMFS >5 was 8.66 (3.57-21.02) if mutans Streptococci ≥ 10³ CFU and 2.11 (0.96-4.64) if Lactobacilli ≥ 10³ CFU.
This data allow us to conclude that hygiene habits and dental visits are not associated with high levels of cariogenic bacteria, but high scores of DMFS were found in adolescents with high levels of mutans Streptococci and lower parents' education.
评估青少年的口腔健康状况与致龋菌水平之间的关系，以及行为和社会人口背景对细菌水平的影响。
研究设计：对 13 岁青少年（女性 112 名，男性 78 名，共 190 名）进行了一项基于人群的横断面研究。根据世界卫生组织（WHO）标准，记录龋齿、缺失和补牙的数量（DMFS）、菌斑和卫生指数。采集无菌容器中的唾液样本，然后在米氏乳杆菌放线杆菌（MSB）琼脂上培养分析变形链球菌，在曼罗加沙普（MRS）琼脂上培养分析乳杆菌。细菌水平以每毫升唾液形成的菌落形成单位数（CFU/ml）表示。采用非条件逻辑回归估计变形链球菌和乳杆菌水平与口腔健康之间的关联，并用比值比（OR）和 95%置信区间（95%CI）表示。
53.2%的青少年未检出变形链球菌，但有 22.6%的青少年的变形链球菌水平≥10³ CFU/ml。对于乳杆菌，这些值分别为 43.7%和 34.7%。在校正性别和社会阶层后，如果变形链球菌≥10³ CFU/ml，DMFS>5的 OR（95%CI）为 8.66（3.57-21.02），如果乳杆菌≥10³ CFU/ml，OR 为 2.11（0.96-4.64）。
本数据表明，卫生习惯和口腔就诊与高致龋菌水平无关，但在变形链球菌水平较高且父母受教育程度较低的青少年中，DMFS 评分较高。
