Niedzielski Przemysław, Mleczek Mirosław, Siwulski Marek, Gąsecka Monika, Kozak Lidia, Rissmann Iwona, Mikołajczak Patrycja
a Department of Analytical Chemistry, Faculty of Chemistry , Adam Mickiewicz University in Poznań , Poznań, Poland.
J Environ Sci Health B. 2014;49(12):929-37. doi: 10.1080/03601234.2014.951576.
The aim of the study was to evaluate the possibility of supplementation with inorganic forms of selenium (Na2SeO4 and Na2SeO3) in concentrations of 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 and 1.5 mM of three medicinal mushroom species: Agrocybe aegerita, Hericium erinaceus and Ganoderma lucidum. Tested mushroom species grew in Se additions of 0-0.6 mM (A. aegerita and H. erinaceus), while growth of G. lucidum bodies was observed for 0-0.8 mM. For the latter mushroom species, the total Se content was the highest. Content of Seorg was diverse; for control bodies it was the highest for G. lucidum (only organic forms were present), lower for A. aegerita (84% organic forms) and the lowest for H. erinaceus (56% organic forms). Accumulation of Se(IV) was generally significantly higher than Se(VI) for all tested mushroom species. There was no significant decrease of A. aegerita or G. lucidum biomass with the exception of G. lucidum bodies growing under 0.8 mM of Se species addition (15.51 ± 6.53 g). Biomass of H. erinaceus bodies was the highest under 0.2 (197.04 ± 8.73 g), control (191.80 ± 6.06 g) and 0.1 mM (185.04 ± 8.73 g) of both inorganic salts. The addition to the medium of Se salts brought about macroscopic changes in the fruiting bodies of the examined mushrooms. Concentrations exceeding 0.4 mM caused diminution of carpophores or even their total absence. In addition, colour changes of fruiting bodies were also recorded. At Se concentrations of 0.4 and 0.6 mM, A. aegerita fruiting bodies were distinctly lighter and those of H. erinaceus changed colour from purely white to white-pink.
本研究的目的是评估添加浓度为0、0.1、0.2、0.4、0.6、0.8、1.0和1.5 mM的无机形式硒（Na2SeO4和Na2SeO3）对三种药用蘑菇（皱环球盖菇、猴头菇和灵芝）的影响。受试蘑菇物种在添加0 - 0.6 mM硒的条件下生长（皱环球盖菇和猴头菇），而灵芝子实体在添加0 - 0.8 mM硒的条件下生长。对于后一种蘑菇物种，总硒含量最高。有机硒含量各不相同；对于对照子实体，灵芝的有机硒含量最高（仅存在有机形式），皱环球盖菇较低（84%为有机形式），猴头菇最低（56%为有机形式）。对于所有受试蘑菇物种，硒（IV）的积累通常显著高于硒（VI）。除了在添加0.8 mM硒物种条件下生长的灵芝子实体（15.51 ± 6.53 g）外，皱环球盖菇或灵芝的生物量没有显著下降。在添加0.2 mM（197.04 ± 8.73 g）、对照（191.80 ± 6.06 g）和0.1 mM（185.04 ± 8.73 g）两种无机盐的条件下，猴头菇子实体的生物量最高。向培养基中添加硒盐导致受试蘑菇子实体出现宏观变化。浓度超过0.4 mM会导致子实体变小甚至完全消失。此外，还记录到了子实体的颜色变化。在硒浓度为0.4和0.6 mM时，皱环球盖菇子实体明显变浅，而猴头菇子实体颜色从纯白色变为白粉色。
