Department of Bioengineering, University of Utah, James LeVoy Sorenson Molecular Biotechnology Building (SMBB), 36 S. Wasatch Drive, Rm. 3100, Salt Lake City, UT 84117, USA.
Department of Bioengineering, University of Utah, James LeVoy Sorenson Molecular Biotechnology Building (SMBB), 36 S. Wasatch Drive, Rm. 3100, Salt Lake City, UT 84117, USA.
Biomaterials. 2015 Jan;36:33-43. doi: 10.1016/j.biomaterials.2014.08.039. Epub 2014 Oct 11.
Available evidence indicates that pro-inflammatory cytokines produced by immune cells are likely responsible for the negative sequela associated with the foreign body response (FBR) to chronic indwelling implants in brain tissue. In this study a computational modeling approach was used to design a diffusion sink placed at the device surface that would retain pro-inflammatory cytokines for sufficient time to passively antagonize their impact on the FBR. Using quantitative immunohistochemistry, we examined the FBR to such engineered devices after a 16-week implantation period in the cortex of adult male Sprague-Dawley rats. Our results indicate that thick permeable surface coatings, which served as diffusion sinks, significantly reduced the FBR compared to implants either with no coating or with a thinner coating. The results suggest that increasing surface permeability of solid implanted devices to create a diffusion sink can be used to reduce the FBR and improve biocompatibility of chronic indwelling devices in brain tissue.
现有证据表明，免疫细胞产生的促炎细胞因子可能是导致慢性脑内留置植入物异物反应（FBR）相关负面后果的原因。在这项研究中，我们采用计算建模方法设计了一种置于器械表面的扩散吸收剂，使其能够在足够长的时间内保留促炎细胞因子，从而被动拮抗它们对 FBR 的影响。我们通过定量免疫组织化学的方法，在成年雄性 Sprague-Dawley 大鼠皮质中植入此类工程器械 16 周后，研究了该器械的 FBR。我们的结果表明，与无涂层或较薄涂层的植入物相比，作为扩散吸收剂的厚、可渗透表面涂层显著降低了 FBR。结果表明，增加固体植入器械的表面渗透性以创建扩散吸收剂，可以用于降低 FBR，并提高脑内慢性留置器械的生物相容性。
