兔、大鼠和小鼠空肠黏膜的6-磷酸果糖-1-激酶同工酶

6-Phosphofructo-1-kinase isoenzymes of the jejunal mucosa of rabbit, rat and mouse.

Khoja S M, Abuelgassim A O

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

Comp Biochem Physiol B. 1989;94(2):269-75. doi: 10.1016/0305-0491(89)90344-1.

Abstract

6-Phosphofructo-1-kinase (PFK) isoenzymes were studied in the jejunal mucosa of rabbit, rat and mouse. 2. The rat mucosal enzyme was found to be very similar to, although not identical with, the mouse mucosal enzyme, as the physical and regulatory properties of these two enzymes were nearly similar except that the immunological studies were dissimilar. 3. PFK prepared from rabbit mucosa showed different and distinct properties from the rat and mouse mucosal PFK when studied by (NH4)2SO4-precipitation, polyacrylamide gel electrophoresis, immunological cross-reactivity and regulatory properties. 4. The difference between the rabbit enzyme and the rat or mouse enzymes is suggested to be due to the lower rate of glycolysis observed in the rabbit jejunal mucosa as the total enzyme activities of the rabbit were found to be less than half of those activities of the rat and mouse mucosa. 5. The dissimilarities among the species in mucosal isoenzymes obtained in the present study are rather expected since the term isoenzyme is now properly reserved for forms that have been shown to be genetically distinct as shown for different tissues in the same species. Such multigenic control does not appear to have been established for the same tissue in different species.

摘要

对兔、大鼠和小鼠空肠黏膜中的6-磷酸果糖-1-激酶（PFK）同工酶进行了研究。2. 发现大鼠黏膜酶与小鼠黏膜酶非常相似，尽管不完全相同，因为这两种酶的物理和调节特性几乎相似，只是免疫学研究结果不同。3. 通过硫酸铵沉淀、聚丙烯酰胺凝胶电泳、免疫交叉反应和调节特性研究发现，从兔黏膜制备的PFK与大鼠和小鼠黏膜PFK表现出不同且独特的特性。4. 兔酶与大鼠或小鼠酶之间的差异被认为是由于在兔空肠黏膜中观察到的糖酵解速率较低，因为发现兔的总酶活性不到大鼠和小鼠黏膜酶活性的一半。5. 本研究中获得的不同物种黏膜同工酶之间的差异在意料之中，因为现在同工酶一词适用于已证明在基因上不同的形式，如同一个物种中不同组织的情况。不同物种同一组织似乎尚未建立这种多基因控制。