Leshina L G, Bulko O V
Tsitol Genet. 2014 Sep-Oct;48(5):36-42.
Plants regenerated from hairy roots and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with different efficiencies. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. Addition of 1 mg/l BA, 0.1 mg/l NAA and 5% sucrose to B5 medium increased regenerative capacity of Vinca roots up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology and short internodes.
已从毛地黄紫色和长春花的毛状根和愈伤组织中获得再生植株。研究发现,毛状根培养中的器官发生在无激素培养基上自发发生,但效率不同。根培养物直接形成芽的频率在毛地黄中高达60%,在长春花中为3.7%。向B5培养基中添加1 mg/l 苄氨基嘌呤、0.1 mg/l 萘乙酸和5%蔗糖可使长春花根的再生能力提高至19.1%。再生植株表现出Ri转基因植物中常见的形态特征。这些特征包括根系的生长和斜向性、芽形成增加、叶片形态改变和节间缩短。