Liu Yan-Long, Gao Xu, Jiang Yang, Zhang Gan, Sun Zi-Cheng, Cui Bin-Bin, Yang Yan-Mei
Department of Colorectal Surgery, The Affiliated Tumor Hospital, Harbin Medical University, No. 150, Haping Rd, Nangang District, Harbin, 150081, Heilongjiang, China.
J Cancer Res Clin Oncol. 2015 Apr;141(4):661-9. doi: 10.1007/s00432-014-1854-5. Epub 2014 Oct 19.
Enhancer of zeste 2 (EZH2), embryonic ectoderm development (EED), and suppressor of zeste 12 homolog (SUZ12), the key component of polycomb repressive complex 2, are of great importance in human cancer pathogenesis. This study was designed to investigate the clinical and prognostic significances of EZH2, EED and SUZ12 in colorectal cancer (CRC) patients.
The expression of EZH2, EED and SUZ12 mRNA was evaluated in 82 primary CRC and paired non-cancerous mucosa samples by qRT-PCR.
We found that overall EZH2, EED and SUZ12 mRNA expression in the CRC tissues was significantly increased than in the non-cancerous tissue (p < 0.05). Increased EZH2, EED and SUZ12 mRNA expression was directly correlated with primary tumor size, regional lymph node metastases, distant metastasis and AJCC stage. Furthermore, CRC patients with higher level of EED, SUZ12 or EZH2 showed a worse disease-free survival (DFS) (p < 0.01). In multivariate analysis, the increased EZH2 expression may be a risk factor for the patients' 3-year DFS (HR 2.517; 95% CI 1.104, 5.736; p = 0.028). Furthermore, the k-means cluster analysis showed that high mRNA expression of EED, SUZ12 and EZH2 was significantly correlated with the aggressive clinical behavior and poor prognosis.
High expression of EED, SUZ12 and EZH2 might contribute to the CRC development/progression.
多梳抑制复合物2的关键成分zeste 2增强子（EZH2）、胚胎外胚层发育蛋白（EED）和zeste 12同源抑制因子（SUZ12）在人类癌症发病机制中具有重要意义。本研究旨在探讨EZH2、EED和SUZ12在结直肠癌（CRC）患者中的临床及预后意义。
采用qRT-PCR检测82例原发性CRC及配对的癌旁黏膜组织中EZH2、EED和SUZ12 mRNA的表达。
我们发现，CRC组织中EZH2、EED和SUZ12 mRNA的总体表达明显高于癌旁组织（p < 0.05）。EZH2、EED和SUZ12 mRNA表达增加与原发肿瘤大小、区域淋巴结转移、远处转移及美国癌症联合委员会（AJCC）分期直接相关。此外，EED、SUZ12或EZH2水平较高的CRC患者无病生存期（DFS）较差（p < 0.01）。多因素分析显示，EZH2表达增加可能是患者3年DFS的危险因素（HR 2.517；95% CI 1.104，5.736；p = 0.028）。此外，k均值聚类分析表明，EED、SUZ12和EZH2的高mRNA表达与侵袭性临床行为及不良预后显著相关。
EED、SUZ12和EZH2的高表达可能促进CRC的发生/发展。
