Isolation of a thymidine kinase negative mutant of Nocardia lactamdurans.
作者信息
Darland G, Rosenbach M, Kaplan L
机构信息
Department of Fermentation Microbiology, Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065.
出版信息
FEMS Microbiol Lett. 1989 Nov;53(1-2):229-32. doi: 10.1016/0378-1097(89)90396-0.
PMID:2533129
Abstract
By selection for resistance to fluorodeoxyuridine in a fluorouracil/fluorouridine resistant background, we have isolated a thymidine kinase negative mutant of Nocardia lactamdurans. This strain is characterized by the inability to incorporate exogenous [2-14C]-thymidine into DNA. The incorporation of radioactive thymine is similarly reduced even in the presence of deoxyadenosine. This phenotype is readily explained by the inability to detect the enzyme thymidine kinase in crude extracts.
摘要
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