Lorenzen Wolfram, Bozhüyük Kenan A J, Cortina Niña S, Bode Helge B
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
Cluster of Excellence Macromolecular Complexes, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
J Lipid Res. 2014 Dec;55(12):2620-33. doi: 10.1194/jlr.M054593. Epub 2014 Oct 20.
Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.
通过超高效(UP)液相色谱-质谱联用技术对细菌的全细胞脂质提取物进行分析,能够全面测定相应生物体中存在的脂质分子种类。这些数据有助于推断其代谢潜力,并创建脂质谱,直观呈现生物体对内部和外部条件变化的反应。在此,我们描述:i)一种快速反相超高效液相色谱-电喷雾质谱方法,适用于检测和测定从单酰甘油磷酸乙醇胺到甘油三酯等各种极性的全细胞脂质提取物中的单个脂质;ii)对营养型黄色粘球菌DK1622细胞中广泛的脂质分子种类的首次概述;iii)在α-羟基化脂肪酸、鞘脂和醚脂生物合成受损的选定突变体中它们相对组成的变化;以及iv)首次报道在黄色粘球菌中存在神经酰胺磷酸肌醇,这种脂质种类以前仅在真核生物中发现。