Guo Li-Li, Wang Jie, Lin Fei, He Yong-Xia
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Sep;34(9):1125-9.
To explore the effect of Danlou Tablet (DT) on arrhythmia model rats induced by transient myocardial ischemia/reperfusion (I/R).
Totally 45 healthy Wistar rats were randomly divided into 3 groups, the sham-operation group, the model group, and the DT group, 15 in each group. Rats in the sham-operation group and the model group were administered with distilled water by gastrogavage at the daily dose of 0.1 mL/kg. Rats in the DT group was administered with 0.53 g/mL DT suspension by gastrogavage at the daily dose of 0.1 mL/kg. All medication was lasted for 10 successive days. The myocardial I/R experiment was performed at 1 h after the last gastrogavage. ECG was performed before ligation and at I/R. The jugular arterial blood pressure of all rats was measured during the whole course. ST segment changes were observed at each time point of I/R. The ventricular fibrillation, the premature ventricular, the number and the duration of ventricular tachycardia within 30 min reperfusion were also observed. Activities of Na(+)-K+ ATPase and Ca2+ ATPase in the myocardium homogenate were detected as well.
The jugular arterial blood pressure and the heart rate were slightly lower in the DT group than in the model group, but with no statistical difference (P > 0.05). Compared with the sham-operation group, the degree of ST segment was obviously elevated in the model group at 0, 5, and 7 min (P < 0.05). It was significantly lower in the DT group than in the model group (P < 0.01). ST seg ment was more elevated at 5 min than at 0 min in the model group, but the degree of ST segment elevation was still obviously lower in the DT group than in the model group (P < 0.05). There was no statistical difference in the degree of ST segment elevation at 7 min between the two groups (P > 0.05). At 0 min when the decrement of ST segment exceeded one half the ischemia, there was no statistical difference in the degree of myocardial ischemia between the model group and the DT group (P > 0.05). Compared with the model group, the incidence of fatal and nonfatal ventricular fibrillation, the frequency and duration of ventricular tachycardia and premature ventricular beats were obviously lessened, and activities of Na(+)-K+ ATPase and Ca(2+)-ATPase increased (all P < 0.05).
DT could significantly protect arrhythmias induced by transient I/R. Its effect might be related to lowering the degree of myocardial ischemia, and increasing ion transport channel related enzyme activities.
探讨丹蒌片（DT）对短暂性心肌缺血/再灌注（I/R）诱导的心律失常模型大鼠的影响。
将45只健康Wistar大鼠随机分为3组，即假手术组、模型组和DT组，每组15只。假手术组和模型组大鼠以0.1 mL/kg的日剂量灌胃蒸馏水。DT组大鼠以0.1 mL/kg的日剂量灌胃0.53 g/mL DT混悬液。所有药物连续给药10天。在末次灌胃后1小时进行心肌I/R实验。在结扎前和I/R时进行心电图检查。在整个过程中测量所有大鼠的颈总动脉血压。在I/R的每个时间点观察ST段变化。还观察了再灌注30分钟内的心室颤动、室性早搏、室性心动过速的次数和持续时间。同时检测心肌匀浆中Na(+)-K+ ATP酶和Ca2+ ATP酶的活性。
DT组颈总动脉血压和心率略低于模型组，但无统计学差异（P>0.05）。与假手术组相比，模型组在0、5和7分钟时ST段抬高程度明显升高（P<0.05）。DT组明显低于模型组（P<0.01）。模型组5分钟时ST段抬高程度高于0分钟，但DT组ST段抬高程度仍明显低于模型组（P<0.05）。两组在7分钟时ST段抬高程度无统计学差异（P>0.05）。在0分钟时，当ST段下降超过缺血的一半时，模型组和DT组心肌缺血程度无统计学差异（P>0.05）。与模型组相比，致命性和非致命性心室颤动的发生率、室性心动过速和室性早搏的频率及持续时间明显降低，Na(+)-K+ ATP酶和Ca(2+)-ATP酶活性增加（均P<0.05）。
DT能显著保护短暂性I/R诱导的心律失常。其作用可能与降低心肌缺血程度、增加离子转运通道相关酶活性有关。
