Rostampour Mohammad, Ghaffari Arghavan, Salehi Peyman, Saadat Farshid
Cellular and Molecular Research Center, Gilan University of Medical Sciences, Rasht, Iran; Department of Physiology, Gilan University of Medical Sciences, Rasht, Iran.
Medical faculty of Gilan University of Medical Sciences, Rasht, Iran.
Basic Clin Neurosci. 2014 Summer;5(3):199-204.
Regarding chronic nature of epilepsy and its side effects and to access the effective treatment procedures, herbal medicine has received remarkable interest. The aim of this study was to determine the anticonvulsant effects of hydro-alcoholic extract of Anethum graveolens seed on pentylenetetrazol (PTZ) -induced seizure in male mice.
Fifty-six albino male mice were divided randomly into seven groups including the negative control (saline), positive control (Phenobarbital) and treatment groups using different doses of hydro-alcoholic extract of Anethum graveolens seed (50, 100, 300, 500 and 1000 mg/ kg). To provoke convulsion, PTZ was injected to all groups and initiation time of myoclonic and tonic-clonic seizures as well as surveillance after 24 h were measured.
The results indicated that hydro-alcoholic extract of Anethum graveolens seed (AGS) delayed the initiation time of myoclonic and tonic-clonic seizures in comparison with saline group. The latency was considerable for myoclonic and tonic-clonic seizures at all above mentioned doses of AGS extract except for the lowest one. Moreover, the protective effect of AGS extract against mortality was statistically significant at all doses except for 50 mg/kg.
As the hydro-alcoholic extract of AGS showed an appropriate response in experimental model of convulsion, it might be considered as an adjuvant therapy with other traditional antiepileptic medications.
鉴于癫痫的慢性性质及其副作用，为了探寻有效的治疗方法，草药受到了广泛关注。本研究的目的是确定莳萝籽水醇提取物对雄性小鼠戊四氮（PTZ）诱导惊厥的抗惊厥作用。
将56只白化雄性小鼠随机分为七组，包括阴性对照组（生理盐水）、阳性对照组（苯巴比妥）和使用不同剂量莳萝籽水醇提取物（50、100、300、500和1000毫克/千克）的治疗组。向所有组注射PTZ以诱发惊厥，并测量肌阵挛和强直阵挛性惊厥的起始时间以及24小时后的监测情况。
结果表明，与生理盐水组相比，莳萝籽水醇提取物（AGS）延迟了肌阵挛和强直阵挛性惊厥的起始时间。除最低剂量外，上述所有剂量的AGS提取物对肌阵挛和强直阵挛性惊厥的潜伏期都相当可观。此外，除50毫克/千克剂量外，AGS提取物在所有剂量下对死亡率的保护作用在统计学上均具有显著意义。
由于AGS的水醇提取物在惊厥实验模型中显示出适当的反应，它可能被视为与其他传统抗癫痫药物的辅助治疗方法。
