[肝素对纤溶酶（原）的蛋白水解和纤维蛋白溶解活性及纤维蛋白凝块溶解的影响]

[The effect of heparin on the proteolytic and fibrinolytic activity on plasmin(ogen) and fibrin clot lysis].

作者信息

Kudinov S A, Kolesnik L A, Makogonenko E M, Shuliakovskaia T A

出版信息

Biokhimiia. 1989 Nov;54(11):1881-7.

Abstract

The effect of heparin on the proteolytic and fibrinolytic activities of plasmin and plasminogen was studied. Heparin at a concentration of 6.3.10(-6) M did not change the caseinolytic activity of plasmin and plasminogen stimulated by streptokinase but suppressed their fibrinolytic activity. At concentrations from 2.10(-8) to 0.5.10(-6) M heparin increased, whereas at 1.10(-6)-4.10(-6) M reduced the time of desAAfibrin clot half-lysis by plasmin. Within the concentration range of 2.10(-8) to 4.10(-6) M heparin did not change the time of the clot half-lysis by glu-plasminogen and slightly decreased the time of fibrin clot half-lysis by lys-plasminogen in the presence of the tissue activator. It was supposed that heparin inhibits the fibrinolytic effect of plasmin by way of formation of complexes with plasmin and reduction of plasmin specificity to the solid phase substrate, i. e., polymeric fibrin.

摘要

研究了肝素对纤溶酶和纤溶酶原的蛋白水解及纤维蛋白溶解活性的影响。浓度为6.3×10⁻⁶ M的肝素不会改变链激酶刺激的纤溶酶和纤溶酶原的酪蛋白溶解活性，但会抑制它们的纤维蛋白溶解活性。在2×10⁻⁸至0.5×10⁻⁶ M的浓度范围内，肝素会延长，而在1×10⁻⁶至4×10⁻⁶ M时会缩短纤溶酶使去氨基纤维蛋白凝块半溶解的时间。在2×10⁻⁸至4×10⁻⁶ M的浓度范围内，肝素不会改变谷氨酰胺纤溶酶原使凝块半溶解的时间，并且在组织激活剂存在的情况下，会略微缩短赖氨酸纤溶酶原使纤维蛋白凝块半溶解的时间。据推测，肝素通过与纤溶酶形成复合物并降低纤溶酶对固相底物即聚合纤维蛋白的特异性，来抑制纤溶酶的纤维蛋白溶解作用。