Rodrigo José A, Alieva Tatiana
Opt Lett. 2014 Oct 1;39(19):5634-7. doi: 10.1364/OL.39.005634.
Partially coherent illumination provides significant advantages such as speckle-free imaging and enhanced optical sectioning in optical microscopy. The knowledge of the spatial and temporal coherence is crucial to obtain accurate quantitative phase imaging (QPI) of specimens such as live cells, micrometer-sized particles, etc. In this Letter, we propose a novel technique for illumination coherence engineering. It is based on a DMD projector providing fast switchable both multi-wavelength and spatial coherence design. Its performance is experimentally demonstrated for QPI with different spatial coherence states.
部分相干照明在光学显微镜中具有显著优势,如无散斑成像和增强光学切片。了解空间和时间相干性对于获取活细胞、微米级颗粒等样本的准确定量相位成像(QPI)至关重要。在本信函中,我们提出了一种用于照明相干工程的新技术。它基于数字微镜器件(DMD)投影仪,可提供多波长和空间相干设计的快速切换。通过实验证明了其在不同空间相干状态下用于QPI的性能。