Guo Huilin, Fang Wei, Li Yingjie, Ke Jin, Deng Mohong, Meng Qinggong, Li Jian, Long Xing
State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
J Oral Pathol Med. 2015 Sep;44(8):622-7. doi: 10.1111/jop.12273. Epub 2014 Nov 4.
Hyaluronic acid (HA) injection is widely used in the treatment of temporomandibular joint (TMJ) osteoarthritis (OA). Proteoglycan 4 (PRG4) is another joint lubricant that protects surface of articular cartilage. But few studies had explored the role of HA in regulation of PRG4 expression in TMJ OA. In this study, the effects of HA on the expression of PRG4 in osteoarthritic TMJ synovial cells were investigated in hypoxia, which was similar to the TMJ physiologically.
Synovial cells were isolated from the TMJ OA patients and were treated with or without HA under normoxia or hypoxia for indicated time periods. The proliferation of synovial cells was measured using Cell Counting Kit-8 (CCK-8). The gene expression of HAS2, VEGF, and PRG4 was detected by quantitative real-time PCR, and the secretion of PRG4 and VEGF was assayed by enzyme-linked immunosorbent assay (ELISA). Immunofluorescence was used to examine the protein expression of hypoxia-induced factor-1α (HIF-1α).
Hyaluronic acid markedly increased the proliferation of osteoarthritic synovial cells in hypoxia. The expression of HAS2 and PRG4 mRNA of osteoarthritic synovial cells under hypoxia was enhanced by HA treatment. However, HA had no effect on reducing the VEGF and HIF-1α expression in synovial cells in hypoxia.
Hyaluronic acid could promote the expression of HAS2 and PRG4, but could not modulate HIF-1α and VEGF expression of TMJ osteoarthritic synovial cells in hypoxia.
透明质酸（HA）注射广泛应用于颞下颌关节（TMJ）骨关节炎（OA）的治疗。蛋白聚糖4（PRG4）是另一种保护关节软骨表面的关节润滑剂。但很少有研究探讨HA在TMJ OA中对PRG4表达的调节作用。在本研究中，在类似于TMJ生理状态的缺氧条件下，研究了HA对骨关节炎TMJ滑膜细胞中PRG4表达的影响。
从TMJ OA患者中分离滑膜细胞，并在常氧或缺氧条件下用或不用HA处理指定时间段。使用细胞计数试剂盒-8（CCK-8）测量滑膜细胞的增殖。通过定量实时PCR检测HAS2、VEGF和PRG4的基因表达，并通过酶联免疫吸附测定（ELISA）测定PRG4和VEGF的分泌。免疫荧光用于检测缺氧诱导因子-1α（HIF-1α）的蛋白表达。
透明质酸在缺氧条件下显著增加了骨关节炎滑膜细胞的增殖。HA处理增强了缺氧条件下骨关节炎滑膜细胞中HAS2和PRG4 mRNA的表达。然而，HA对缺氧条件下滑膜细胞中VEGF和HIF-1α的表达没有影响。
透明质酸可促进HAS2和PRG4的表达，但在缺氧条件下不能调节TMJ骨关节炎滑膜细胞中HIF-1α和VEGF的表达。
Zotero 插件