在中国患者中，Ku70和ATM的下调与结直肠癌的不良预后相关。
Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients.
作者信息
Lu Yuanfang, Gao Jingyan, Lu Yuanming
机构信息
Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China ; Department of Clinical Research Center, Affiliated 2nd Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China ; Department of Human Anatomy and Histo-Embryology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
出版信息
Onco Targets Ther. 2014 Oct 24;7:1955-61. doi: 10.2147/OTT.S67814. eCollection 2014.
BACKGROUND
Double-strand DNA breaks (DSBs) are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM) in colorectal cancer (CRC), we examined the expression levels and patterns of Ku70 and ATM in CRC samples.
METHODS
Expression and coexpression of Ku70 and ATM were investigated by using real-time quantitative polymerase chain reaction assays and confirmed further with fluorescent immunohistochemistry in CRC and pericancerous samples from 112 Chinese patients.
RESULTS
Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate.
CONCLUSION
Downregulated Ku70 and ATM were associated with poor disease-free survival. Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC.
背景
双链DNA断裂（DSB）是致癌作用的关键因素。DSB的必要修复对于维持正常细胞分裂至关重要。为了探讨结直肠癌（CRC）中蛋白质Ku70和共济失调毛细血管扩张突变蛋白（ATM）的双链DNA修复表达改变之间的关系，我们检测了CRC样本中Ku70和ATM的表达水平及模式。
方法
采用实时定量聚合酶链反应分析研究Ku70和ATM的表达及共表达情况，并通过荧光免疫组织化学在112例中国患者的CRC及癌旁样本中进一步证实。
结果
在CRC样本中发现Ku70和ATM均呈低表达模式，且与肿瘤淋巴结转移晚期显著相关，并降低了5年总生存率。
结论
Ku70和ATM下调与无病生存期差有关。Ku70和ATM表达缺失可能作为预测CRC患者预后不良的生物标志物。
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