琼脂糖水凝胶诱导的Sp2/0、A549、MCF-7细胞系三维培养的培养阶段、细胞毒性和蛋白表达
Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.
作者信息
Ravi Maddaly, Kaviya S R, Paramesh V
机构信息
Department of Human Genetics, Faculty of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai, 600116, India.
出版信息
Cytotechnology. 2016 May;68(3):429-41. doi: 10.1007/s10616-014-9795-z. Epub 2014 Nov 5.
Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied. Also, the 3D aggregates of the three cell lines were reverted into 2D cultures and the protein profile differences among the 2D, 3D and revert cultures were studied. The analysis of protein profile differences using UniProt data base further augment the usefulness of agarose hydrogels for obtaining 3D cell cultures.
细胞培养技术正在迅速发展，一个重要的方向是在三维条件下培养细胞。我们证明了琼脂糖水凝胶在获得三种细胞系A549、MCF-7和Sp2/0的三维聚集体方面的实用性。研究了培养阶段的差异以及对顺铂诱导的细胞毒性的敏感性。此外，将这三种细胞系的三维聚集体恢复为二维培养，并研究了二维、三维和恢复培养之间的蛋白质谱差异。使用UniProt数据库对蛋白质谱差异进行分析，进一步增强了琼脂糖水凝胶用于获得三维细胞培养的实用性。
Zotero 插件