ARFGAP1与肝细胞中的脂滴动态相关。
ARFGAP1 is dynamically associated with lipid droplets in hepatocytes.
作者信息
Gannon Joan, Fernandez-Rodriguez Julia, Alamri Hussam, Feng Shi Bo, Kalantari Fariba, Negi Sarita, Wong Amy H Y, Mazur Alexander, Asp Lennart, Fazel Ali, Salman Ayat, Lazaris Anthoula, Metrakos Peter, Bergeron John J M, Nilsson Tommy
机构信息
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
The Centre for Cellular Imaging at the University of Gothenburg, Gothenburg, Sweden.
出版信息
PLoS One. 2014 Nov 14;9(11):e111309. doi: 10.1371/journal.pone.0111309. eCollection 2014.
The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.
ARF GTP酶激活蛋白1（ARFGAP1）主要与高尔基体潴泡膜的胞质侧结合，在那里它参与COPI和网格蛋白包被小泡的形成。在本研究中，我们发现，在培养细胞中添加油酸后，ARFGAP1会短暂地与脂滴结合。此外，添加环磷酸腺苷会使ARFGAP1从脂滴转移至高尔基体，并且ARFGAP1的过表达和敲低会影响脂滴的形成。对人肝组织的检测显示，在稳态下，ARFGAP1在部分而非全部肝细胞中与脂滴相关联。
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