Gomes Jacinto, Inácio João
Instituto Nacional de Investigação Agrária e Veterinária IP, Lisbon, Portugal.
Methods Mol Biol. 2015;1247:175-82. doi: 10.1007/978-1-4939-2004-4_13.
Tropical theileriosis is a tick-borne disease responsible for important health problems in cattle, caused by the hemoprotozoan Theileria annulata. Traditionally, detection of Theileria pathogens in infected animals requires the microscopic examination of stained-blood smears and serological methods. Molecular diagnostic assays have been developed for the detection of Theileria parasites, including PCR-based and reverse line blotting approaches, but these methods usually demand qualified personnel, complex instrumentation, and expensive materials. Loop-mediated isothermal amplification (LAMP) can facilitate the design of molecular assays independent of the use of sophisticated equipment. In this chapter we describe the application of two molecular assays for the direct detection of T. annulata in bovine blood samples, based in real-time PCR and LAMP, both targeting the Tams1-encoding gene of this parasite.
热带泰勒虫病是一种由血液原虫环形泰勒虫引起的蜱传疾病，会给牛群带来严重的健康问题。传统上，检测感染动物体内的泰勒虫病原体需要对染色血涂片进行显微镜检查以及采用血清学方法。已经开发出了用于检测泰勒虫寄生虫的分子诊断检测方法，包括基于聚合酶链反应（PCR）的方法和反向线印迹法，但这些方法通常需要专业人员、复杂的仪器设备和昂贵的材料。环介导等温扩增技术（LAMP）有助于设计无需使用精密设备的分子检测方法。在本章中，我们描述了两种基于实时PCR和LAMP的分子检测方法在直接检测牛血样中环形泰勒虫的应用，这两种方法均靶向该寄生虫的Tams1编码基因。
