Shen Qi-Ying, Fang Lei, Wu Hui-Mei, He Fang, Ding Pei-Shan, Liu Rong-Yu
Department of Pulmonary, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Department of Anesthesiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Respirology. 2015 Feb;20(2):258-63. doi: 10.1111/resp.12439. Epub 2014 Nov 17.
Repeated inhalation of sevoflurane (SVF) can benefit asthmatic patients by bronchodilation. However, the impact of repeated inhalation of SVF on allergic airway inflammation has not been clarified. This study was aimed at investigating the effects of repeated inhalation of SVF on airway inflammation in mice.
Female C57BL/6 mice were sensitized with ovalbumin (OVA) and treated by inhalation with SVF or vehicle daily for seven consecutive days, immediately followed by OVA challenge. Airway inflammation was evaluated by counting the numbers of different types of inflammatory infiltrates in bronchoalveolar lavage fluid (BALF), histology, cytokine measurements and mucus production in individual mice.
In comparison with the OVA group, repeated inhalation of SVF significantly reduced the numbers of total cells, eosinophils, lymphocytes, macrophages and neutrophils (P < 0.05 to P < 0.01), and the levels of BALF tumour necrosis factor-α and lung high-mobility group box 1 (P < 0.01), accompanied by elevated levels of BALF interleukin-10 in allergic mice (P < 0.05). Repeat inhalation of SVF decreased the levels of serum OVA-specific immunoglobulin E (IgE) and mitigated allergic airway epithelial goblet cell hyperplasia and mucus hypersecretion in allergic mice (P < 0.01).
Repeated inhalation of SVF inhibits allergic airway inflammation by reducing inflammatory infiltrates, improving the imbalance of cytokine responses and mitigating allergen-specific IgE responses and goblet cell hyperplasia in mice.
反复吸入七氟醚（SVF）可通过支气管扩张使哮喘患者受益。然而，反复吸入SVF对过敏性气道炎症的影响尚未阐明。本研究旨在探讨反复吸入SVF对小鼠气道炎症的影响。
雌性C57BL/6小鼠用卵清蛋白（OVA）致敏，连续7天每天吸入SVF或赋形剂，随后立即进行OVA激发。通过计数支气管肺泡灌洗液（BALF）中不同类型炎性浸润细胞的数量、组织学检查、细胞因子检测以及个体小鼠的黏液分泌来评估气道炎症。
与OVA组相比，反复吸入SVF显著减少了总细胞、嗜酸性粒细胞、淋巴细胞、巨噬细胞和中性粒细胞的数量（P<0.05至P<0.01），以及BALF肿瘤坏死因子-α和肺高迁移率族蛋白B1的水平（P<0.01），同时过敏性小鼠BALF白细胞介素-10水平升高（P<0.05）。反复吸入SVF降低了血清OVA特异性免疫球蛋白E（IgE）水平，并减轻了过敏性小鼠气道上皮杯状细胞增生和黏液分泌亢进（P<0.01）。
反复吸入SVF通过减少炎性浸润、改善细胞因子反应失衡以及减轻小鼠过敏原特异性IgE反应和杯状细胞增生来抑制过敏性气道炎症。
