用于皮肤黑色素瘤检测的时间分辨荧光寿命
Time-resolved fluorescence lifetime for cutaneous melanoma detection.
作者信息
Pires Layla, Nogueira Marcelo Saito, Pratavieira Sebastião, Moriyama Lilian Tan, Kurachi Cristina
机构信息
Universidade de São Paulo, Instituto de Física de São Carlos - IFSC/USP, Av. Trabalhador São Carlense,400 - São Carlos, SP, Brazil.
出版信息
Biomed Opt Express. 2014 Aug 22;5(9):3080-9. doi: 10.1364/BOE.5.003080. eCollection 2014 Sep 1.
Abstract
Melanoma is the most aggressive skin cancer type. It is characterized by pigmented lesions with high tissue invasion and metastatic potential. The early detection of melanoma is extremely important to improve patient prognosis and survival rate, since it can progress to the deadly metastatic stage. Presently, the melanoma diagnosis is based on the clinical analysis of the macroscopic lesion characteristics such as shape, color, borders following the ABCD rules. The aim of this study is to evaluate the time-resolved fluorescence lifetime of NADH and FAD molecules to detect cutaneous melanoma in an experimental in vivo model. Forty-two lesions were analyzed and the data was classified using linear discriminant analysis, a sensitivity of 99.4%, specificity of 97.4% and accuracy of 98.4% were achieved. These results show the potential of this fluorescence spectroscopy for melanoma detection.
摘要
黑色素瘤是最具侵袭性的皮肤癌类型。其特征是具有高组织侵袭性和转移潜力的色素沉着病变。黑色素瘤的早期检测对于改善患者预后和生存率极为重要，因为它可能发展到致命的转移阶段。目前，黑色素瘤的诊断基于对宏观病变特征（如形状、颜色、边界）按照ABCD规则进行临床分析。本研究的目的是评估NADH和FAD分子的时间分辨荧光寿命，以在实验性体内模型中检测皮肤黑色素瘤。分析了42个病变，并使用线性判别分析对数据进行分类，获得了99.4%的灵敏度、97.4%的特异性和98.4%的准确率。这些结果表明这种荧光光谱法在黑色素瘤检测方面具有潜力。
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