Takahashi T, Obata Y, Sekido Y, Hida T, Ueda R, Watanabe H, Ariyoshi Y, Sugiura T, Takahashi T
Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.
Cancer Res. 1989 May 15;49(10):2683-8.
Eighteen small cell lung cancer (SCLC) lines (including nine lines established by this group) as well as 31 tumor samples from 23 SCLC patients were examined for the surface antigen phenotype and the expression and amplification of the myc gene family. The expression of NE-150 neuroendocrine, PE-35 panepithelial and OE-130 epithelial antigens corresponded well with the level of biomarkers of SCLC lines, i.e., the NE-150+/PE-35+/OE-130- phenotype corresponded to classic type, while the other phenotypes such as NE-150+/PE-35-/OE-130- to variant type. In tumor specimens, most classic SCLC (consisting of oat cell type and intermediate cell type, subtype a) showed NE-150+/PE-35+/OE-130- phenotype, while small cell-large cell carcinoma (intermediate cell type, subtype b) expressed various phenotypes. The amplification of the myc gene family was observed in nine out of 18 lines (50%) and five out of 23 patient tumors (22%). Higher levels of expression of either c-myc, N-myc, or L-myc were detected in 16 out of 18 lines (89%) and in five out of six patient tumors (83%), when compared with that of normal or fetal lung tissues. Thus, the higher expression without obvious myc gene amplification was observed. The cell lines and tumors with the amplified myc always expressed their corresponding myc genes. The results suggested that higher levels of expression of the myc gene family may play a significant role in the oncogenesis of SCLC. Amplification and/or high levels of expression of c-myc were observed not only in variant type SCLC lines, but also in classic type lines. Thus, they were not necessarily associated with distinct biomarkers of SCLC lines.
对18个小细胞肺癌（SCLC）细胞系（包括本组建立的9个细胞系）以及来自23例SCLC患者的31个肿瘤样本进行了表面抗原表型及myc基因家族表达与扩增情况的检测。NE - 150神经内分泌抗原、PE - 35全上皮抗原和OE - 130上皮抗原的表达与SCLC细胞系的生物标志物水平相符，即NE - 150 + /PE - 35 + /OE - 130 - 表型对应经典型，而其他表型如NE - 150 + /PE - 35 - /OE - 130 - 对应变异型。在肿瘤标本中，大多数经典SCLC（由燕麦细胞型和中间细胞型，a亚型组成）表现为NE - 150 + /PE - 35 + /OE - 130 - 表型，而小细胞 - 大细胞癌（中间细胞型，b亚型）表达多种表型。在18个细胞系中有9个（50%）以及23例患者肿瘤中有5个（22%）观察到myc基因家族的扩增。与正常或胎儿肺组织相比，在18个细胞系中有16个（89%）以及6例患者肿瘤中有5个（83%）检测到c - myc、N - myc或L - myc的较高表达水平。因此，观察到了无明显myc基因扩增的较高表达情况。myc基因扩增的细胞系和肿瘤总是表达其相应的myc基因。结果表明，myc基因家族的较高表达水平可能在SCLC的肿瘤发生中起重要作用。不仅在变异型SCLC细胞系中，而且在经典型细胞系中均观察到c - myc的扩增和/或高表达水平。因此，它们不一定与SCLC细胞系的特定生物标志物相关。
